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Showing: 601 - 650 of 1932 results

  8. pDG335

    Plasmid
    #100899
    Purpose
    SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100899-plasmid-map-sequence-id-195324

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  9. pYPQ265E6

    Plasmid
    #213469
    Purpose
    Gateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing
    Depositor
    Yiping Qi
    Article
    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:
    Insert
    TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
    Use
    CRISPR; Gateway compatible tada-cda-32aa linker-z…
    Tags
    No
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213469-plasmid-map-sequence-id-426729

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  10. pYPQ265E7

    Plasmid
    #213470
    Purpose
    Gateway entry clone (attL1 & attR5) TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing
    Depositor
    Yiping Qi
    Article
    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:
    Insert
    TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI
    Use
    CRISPR; Gateway compatible tada-cdd-32aa linker-z…
    Tags
    No
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213470-plasmid-map-sequence-id-426727

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  11. pYPQ265E8

    Plasmid
    #213471
    Purpose
    Gateway entry clone (attL1 & attR5) TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing
    Depositor
    Yiping Qi
    Article
    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:
    Insert
    TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI
    Use
    CRISPR; Gateway compatible tada-cdd_v106w-32aa li…
    Tags
    No
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213471-plasmid-map-sequence-id-426730

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  14. pJOG252

    Plasmid
    #80584
    Purpose
    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII
    Depositor
    Johannes Stuttmann
    Article
    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1
    Inserts
    pnos:nptII-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A
    Promoter
    Available since
    Sept. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    80584-plasmid-map-sequence-id-151870

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  15. pJOG253

    Plasmid
    #80585
    Purpose
    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA
    Depositor
    Johannes Stuttmann
    Article
    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1
    Inserts
    pnos:PAT-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A
    Promoter
    Available since
    Sept. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    80585-plasmid-map-sequence-id-151872

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  17. pBUN501

    Plasmid
    #50582
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    zCas9D10A
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Expression
    Mutation
    Cas9D10A nickase, was derived from the zCas9 and …
    Promoter
    AtU6-26p and Ubi1p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50582-plasmid-map-sequence-id-75642

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  18. pHSN501

    Plasmid
    #50589
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    zCas9D10A
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Expression
    Mutation
    Cas9D10A nickase, was derived from the zCas9 and …
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50589-plasmid-map-sequence-id-75662

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  24. pORFE1052

    Plasmid
    #112078
    Purpose
    Overexpression of the AtCAS9 nickase in plant under 2x35S promoter
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 D10A
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized nCas9 D10A S. pyogenes
    Promoter
    2x35S
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112078-plasmid-map-sequence-id-217613

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  25. pCDNA3-hTLR4-VenusFlag

    Plasmid
    #197640
    Purpose
    Human TOLL protein expression in mammalian cells
    Depositor
    Haian Fu
    Insert
    TLR4 (TLR4 Human)
    Use
    Tags
    Venus-Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197640-plasmid-map-sequence-id-391702

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  26. pDEST26-hTLR4-Nluc

    Plasmid
    #197642
    Purpose
    Human TOLL protein expression in mammalian cells
    Depositor
    Haian Fu
    Insert
    TLR4 (TLR4 Human)
    Use
    Tags
    NanoLuciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197642-plasmid-map-sequence-id-392621

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  27. pDEST26-Nluc-hTLR4

    Plasmid
    #197641
    Purpose
    Human TOLL protein expression in mammalian cells
    Depositor
    Haian Fu
    Insert
    TLR4 (TLR4 Human)
    Use
    Tags
    NanoLuciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197641-plasmid-map-sequence-id-392580

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  28. pX335 Mouse 5' Srcap gRNA B

    Plasmid
    #127905
    Purpose
    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene
    Depositor
    Mark Groudine
    Insert
    Srcap gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127905-plasmid-map-sequence-id-249688

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  29. pX335 Mouse 5' Srcap gRNA A

    Plasmid
    #127904
    Purpose
    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene
    Depositor
    Mark Groudine
    Insert
    Srcap gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127904-plasmid-map-sequence-id-249683

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  30. pCMV-ABE-P48R

    Plasmid
    #161816
    Purpose
    Expresses ABE-P48R in mammalian cells
    Depositor
    Sangsu Bae
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    161816-plasmid-map-sequence-id-320046

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  31. pCMV-ABE-P48R-UGI

    Plasmid
    #161817
    Purpose
    Expresses ABE-P48R-UGI in mammalian cells
    Depositor
    Sangsu Bae
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Promoter
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    161817-plasmid-map-sequence-id-320651

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  35. pDEST27-GST-hADAM10

    Plasmid
    #189553
    Purpose
    Protein expression ADAM10 in mammalian cells.
    Depositor
    Haian Fu
    Insert
    ADAM metallopeptidase domain 10 (ADAM10 Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    189553-plasmid-map-sequence-id-384138

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  37. pSCM167.2-Venus-VF-hADAM10

    Plasmid
    #189552
    Purpose
    Protein expression ADAM10 in mammalian cells.
    Depositor
    Haian Fu
    Insert
    ADAM metallopeptidase domain 10 (ADAM10 Human)
    Use
    Tags
    VF
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189552-plasmid-map-sequence-id-374370

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  41. pLenti-Arch-EEN

    Plasmid
    #45189
    Purpose
    Arch voltage indicator with double mutations D95N-D106E (Arch-EEN), with faster kinetics and greater fluorescence dynamic range than Arch-D95N
    Depositor
    Mark Schnitzer
    Article
    Gong et al PLoS One. 2013 Jun 19;8(6):e66959.
    Insert
    enhanced Archaerhodopsin-3
    Use
    Lentiviral
    Tags
    eYFP
    Expression
    Mammalian
    Mutation
    D95N, D106E
    Promoter
    CamKIIa
    Available since
    July 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    45189-plasmid-map-sequence-id-63108

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Showing: 601 - 650 of 1932 results