pEntry_SARS-CoV-2-Spike
(Plasmid #168891)

• Purpose: Gateway-compatible Entry vector
• Depositing Lab: Pascal Falter-Braun
• Publication: Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 10.1093/g3journal/jkad105.

Backbone

• Vector backbone: pENTR223.1*SfiI
• Backbone size w/o insert (bp): 2800
• Vector type: Gateway-compatible Entry vector

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SARS-CoV-2-Spike
• Alt name: Spike (S)
• Species: Other
• Insert Size (bp): 3822
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SfiI (unknown if destroyed)
• 3′ cloning site: SfiI (unknown if destroyed)
• 5′ sequencing primer: M13 uni (-21)
• 3′ sequencing primer: M13 rev (-29)

Resource Information

• Supplemental Documents:
  • pEntry_SARS-CoV-2-Spike.gb
• A portion of this plasmid was derived from a plasmid made by: Synthesized by Twist Bioscience

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).