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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 8634 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDM8
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4356
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Tetracycline, 100 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Unknown
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Growth instructionsThe host strain is MC1061/P3 and the bacteria should be cultured in 12.5ug/mL ampicillin and 16ug/mL tetracycline. (supF)
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameICAM-2
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Alt nameCD102
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Alt namepCDIC2.27
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SpeciesH. sapiens (human)
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Insert Size (bp)1100
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GenBank IDNM_000873
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Entrez GeneICAM2 (a.k.a. CD102)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This clone will direct the expression of functional human ICAM-2 in a variety of cell types. This sequence has been deposited in Genbank with accession number X15606. Also see ref: Nature 329, 840-842 (1987)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Human ICAM-2 was a gift from Timothy Springer (Addgene plasmid # 8634 ; http://n2t.net/addgene:8634 ; RRID:Addgene_8634) -
For your References section:
Functional cloning of ICAM-2, a cell adhesion ligand for LFA-1 homologous to ICAM-1. Staunton DE, Dustin ML, Springer TA. Nature 1989 May 4;339(6219):61-4. 10.1038/339061a0 PubMed 2497351
Map uploaded by the depositor.