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Plasmid#86890Purposeexpress D70A variant PvuRts1I in ER2566 strainDepositorInsertPvuRts1I (pvuRts1I )
UseTagsHisExpressionBacterialMutationD70APromoterT7Available sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pST50Trc4-HPCNDHFR
Plasmid#63998PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc2-HPCNDHFR
Plasmid#63962PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-HPCNDHFR
Plasmid#63980PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-FLAGHISNDHFR
Plasmid#63983PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & FLAG; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-HPCNDHFR
Plasmid#63944PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pHR-TRE3G-dCas9-HaloTag
Plasmid#169448Purposeexpression of dCas9-HaloTagDepositorInsertdCas9-HaloTag
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Pol-II WT
Plasmid#35175DepositorInsertRPBI (POLR2A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
BSP605
Plasmid#122250Purposeexpression vector for mCerulean under EFT3 promoterDepositorInsertmCerulean
UseTagsExpressionWormMutationPromoterEFT3Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7-gRNA
Plasmid#46759PurposegRNA cloning vector for in vitro transcription of target gRNADepositorInsertgRNA core
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAC-lacZ
Plasmid#13422DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/LoxTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCS2-nCas9n
Plasmid#47929Purposeexpression of an optimized Cas9 for genome-editing in zebrafishDepositorInsertnls-zcas9-nls
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSCV PIG (Puro IRES GFP empty vector)
Plasmid#21654PurposeRetroviral backbone for cloning and gene expression. Select with puromycin or GFP.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsGFP and IRESExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pU6-As-crRNA
Plasmid#78956PurposeCloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
ZipGFP11_TEV
Plasmid#81243PurposeZipped GFP11 with TEV cleavage sequenceDepositorInsertzipped GFP11 with TEV cleavage sequence
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKSI-1
Plasmid#51725Purposecloning backboneDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHC79
Plasmid#167551PurposeBackbone plasmid to pPM1001, pPM1002, pPM1003, pPM2212 and pPM2213DepositorTypeEmpty backboneUseCosmidTagsExpressionMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only