pDL127
(Plasmid
#43911)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 43911 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRS416
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4898
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDIF1
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SpeciesS. cerevisiae (budding yeast)
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MutationHUG domain mutated VGMRIRQ->AAAAAAA, S59, Q66H
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Entrez GeneDIF1 (a.k.a. YLR437C)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Cla1 (unknown if destroyed)
- 3′ cloning site Xba1 (unknown if destroyed)
- 5′ sequencing primer M13_forward20_primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDL127 was a gift from Stephen Elledge (Addgene plasmid # 43911 ; http://n2t.net/addgene:43911 ; RRID:Addgene_43911) -
For your References section:
Dif1 is a DNA-damage-regulated facilitator of nuclear import for ribonucleotide reductase. Lee YD, Wang J, Stubbe J, Elledge SJ. Mol Cell. 2008 Oct 10;32(1):70-80. doi: 10.1016/j.molcel.2008.08.018. 10.1016/j.molcel.2008.08.018 PubMed 18851834