Addgene

Showing: 2541 - 2560 of 28572 results

Results per page:

Sort results by:

PiggyBac EF1a-Tet-on 3G-Puro U6 BbsI

Plasmid

#159756

Purpose

PiggybacTransposon-based expression control of Tet-on 3G, Puro and Cas13d U6 gRNA backbone

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Type

Empty backbone

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

Plasmid

#221552

Purpose

Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

Insert

gRNA targeting human Rab7A (RAB7A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-IRAK4-mScarlet-P2A-Puro

Plasmid

#221237

Purpose

Stable expression of mouse IRAK4-mScarlet after Lentiviral transduction

Depositor

Jonathan Kagan

Insert

IRAK4 (Irak4 Mouse)

Use

Lentiviral

Tags

mScarlet

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPaint-APEX2-GFP-3xFLAG-PuroR

Plasmid

#221238

Purpose

CRISPaint gene tagging

Depositor

Jonathan Kagan

Insert

APEX2-GFP-3xFLAG

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Xlone Puro SNAP-eGFP-BASU

Plasmid

#218452

Purpose

expresses the SNAP-eGFP-BASU fusion protein via stable PiggyBac integration

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

SNAP-eGFP-BASU

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3GS

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN-X-mPGK-PuroR-SV40 polyA-Z

Plasmid

#218908

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

mPGK-PuroR-SV40 polyA

Use

Tags

Expression

Mammalian

Mutation

297T>C

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-Puro 2xStrep-HPC4-Gb5

Plasmid

#218177

Purpose

Used to make a lenti virus for stable expression of the G protein beta 5 subunit in mammalian cells

Depositor

Barry Willardson

Article

Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

Insert

G beta subunit 5 (GNB5 Human)

Use

Lentiviral

Tags

2x Strep tag and HPC4

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-CMV-MLH1dn-IRES-puroR

Plasmid

#207354

Purpose

Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

MutL homolog 1 (MLH1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Δ754-756 dominant negative mutant

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-RNF2-ngRNA+41_EF1a-puroR

Plasmid

#207358

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

RNF2 +41 ngRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-N1303K-ngRNA+14_EF1a-puroR

Plasmid

#207359

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

N1303K-CFTR +14 ngRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-L227R-ngRNA+15_EF1a-puroR

Plasmid

#207360

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of L227R-CFTR via prime editing and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

L227R-CFTR +15 ngRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SlugCBE-NNG-P2A-puro

Plasmid

#214355

Purpose

Expresses SlugCBE-NNG and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugCBE-NNG and puromycin resistance genes

Use

Tags

Expression

Mammalian

Mutation

Q782R/S888R/L906R/N984S/E1012K/K1016I

Promoter

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-CAG-PlatTET-wpA-pPuroTK

Plasmid

#214125

Purpose

Gene expression by Piggybac transposase in mammalian cells

Depositor

Sho Yoshimatsu

Insert

dCas9-TET

Use

Tags

Expression

Mammalian

Mutation

Codon-optimized for mammalian cells

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Pb459-mU6-sgRNA-EF1a-PuroR

Plasmid

#195507

Purpose

piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

Depositor

Joanna Wysocka

Article

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

Puromycin

Use

CRISPR; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPBpuro-miRFP703-eDHFR(69K6)-cRaf

Plasmid

#184717

Purpose

iRaf

Depositor

Michiyuki Matsuda

Article

Watabe et al Elife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

miRFP703-eDHFR(69K6)-cRaf (RAF1 Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPBpuro-UbC-4493NES(Booster-PKA)

Plasmid

#184711

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al Elife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CMV-GFP-Puro_TAL1-short

Plasmid

#210641

Purpose

Overexpression of TAL1-short

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1-short (TAL1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNKX2-5-T2A-GFP-PGK-Puro

Plasmid

#203607

Purpose

Donor plasmid to create 3' knockin of NKX2-5 with T2A-GFP

Depositor

Matthew Birket

Article

Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

Insert

NKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)

Use

Human targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-V1AUC-DelStem-CD20-Puro

Plasmid

#209758

Purpose

Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1; variant 1 mRNA isoform with mutations to disrupt the uORFs and the stem-loop within the 5'-UTR.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Mutations to disrupt the uORFs and the stem-loop …

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FLAG-puro-mOct4 S351A

Plasmid

#206913

Purpose

Expression of mOct4 S351A mutation

Depositor

Hyonchol Jang

Insert

Oct4 (Pou5f1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S351A

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge