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Plasmid#41553DepositorInsertASC (PYCARD Human)
UseTagsHAExpressionMammalianMutationPromoterCMV immediate-early enhancer/promoterAvailable sinceDec. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-24xGCN4_v4-sfGFP
Plasmid#61058PurposeA direct fusion of sfGFP to the 24xGCN4_v4 peptide arrayDepositorInsert24xGCN4_v4 peptide array and sfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
FUGW-PK-hLC3
Plasmid#61460PurposeExpress pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)DepositorInsertPK-hLC3 (MAP1LC3B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCIBN(deltaNLS)-pmGFP
Plasmid#26867PurposeExpresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membraneDepositorInsertCIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)
UseTagsEGFPExpressionMammalianMutationNLS sequences mutatedPromoterCMVAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
GFP-rab7 DN
Plasmid#12660DepositorInsertRAB7 (Homo sapiens) (RAB7A Human)
UseTagsGFP and HAExpressionMammalianMutationT22NPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)
Plasmid#79620PurposeExpresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cellsDepositorInsertSpCas9
UseTagsPmCDA1 and UGIExpressionMammalianMutationD10A for nickase Cas9PromoterpCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-eNpHR 3.0-EYFP
Plasmid#26971PurposeAAV expression of eNpHR 3.0 fused to EYFP driven by CaMKIIa promoter for optogenetic inhibitionDepositorHas ServiceAAV1 and AAV9InserteNpHR 3.0
UseAAVTagsEYFPExpressionMammalianMutationTrafficking Signal (TS) ER Export SignalPromoterCaMKIIaAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
FH-TET3-pEF
Plasmid#49446PurposeThis plasmid expresses full-length human TET3.DepositorInsertTet methylcytosine dioxygenase 3 (TET3 Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterEF-1alphaAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJL-TRBO
Plasmid#80082PurposeAn improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
FH-Tet2-pEF
Plasmid#41710PurposeThis plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian systemDepositorInserttet methylcytosine dioxygenase 2 (Tet2 Mouse)
UseTagsFLAG and HAExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCalN-ddGFP-A
Plasmid#40290DepositorInsertCalnexin (CANX Human)
UseTagsddGFP-AExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2
Plasmid#81041PurposeMammalian or insect expression for wt LOV2DepositorInsertLOV2
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterCMV, p10Available sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nb-gp41-Halo (MoonTag-Nb-Halo)
Plasmid#128603PurposeExpresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).DepositorInsertNanobody_gp41
UseLentiviralTagsGB1 and HaloExpressionMutationPromoterSFFVAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
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TetO-FUW-cMYC
Plasmid#20324DepositorInsertcMYC (Myc Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
Sfp pet29b C-terminal His Tag
Plasmid#75015PurposeExpresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACPDepositorInsertSfp
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceMay 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Rab5CA(Q79L)
Plasmid#35138DepositorInsertRab5a (RAB5A Human)
UseTagsmCherryExpressionMammalianMutationConstitutively Active- Q79LPromoterCMVAvailable sinceMarch 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/FLAG/HA-DEST DICER
Plasmid#19881DepositorInsertDicer 1, ribonuclease type III (DICER1 Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mGFP-Actin
Plasmid#21948DepositorInsertbetaActin (ACTB Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET mCherry LIC cloning vector (u-mCherry)
Plasmid#29769DepositorTypeEmpty backboneUseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only