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Plasmid#41181DepositorInsertCytochrome C (CYCS Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHAhFMRPiso1
Plasmid#48690PurposeExpresses hFMRP iso 1DepositorInsertFlagHA FMR1 Iso 1 (FMR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
MCP-APEX2
Plasmid#154936PurposeNLS-V5-MCP-APEX2DepositorInsertNLS-V5-MCP-APEX2
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-FUS/TLS-FLAGC
Plasmid#60362PurposePlasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.DepositorInsertFUS (FUS Human)
UseTagsFLAG and GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-BRAF-V600E
Plasmid#15269PurposeRetroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)DepositorInsertBRAF (BRAF Human)
UseRetroviralTagsHis and MycExpressionMammalianMutationValine 600 to Glutamate (mutation has been found …PromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
PresentER-SIINFEKL (GFP)
Plasmid#102944PurposePresentER minigene encoding H2-Kb ligand SIINFEKL (GFP)DepositorInsertSIINFEKL (OVAL Synthetic, Chicken)
UseRetroviralTagsExpressionMammalianMutationOVA257-264PromoterLTRAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-Keap1
Plasmid#28023DepositorInsertKeap1 (KEAP1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-IQGAP1
Plasmid#30112DepositorInsertIQGAP1 (IQGAP1 Human)
UseTagstandem EGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
c-myc-PT3EF1a
Plasmid#92046PurposeExpresses c-Myc in mammalian cellsDepositorInsertC-MYC (MYC Human)
UseTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceJuly 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Peroxisomes-2
Plasmid#54520PurposeLocalization: Peroxisomes, Excitation: 587, Emission: 610DepositorTypeEmpty backboneUseTagsPeroximal Targeting Signal 1 (PTS1) and mCherryExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-14-3-3 epsilon
Plasmid#13273DepositorInsert14-3-3 epsilon (YWHAE Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV/D374Y-hPCSK9
Plasmid#58379PurposeExpresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosisDepositorInsertproprotein convertase subtilisin/kexin type 9 (PCSK9 Human)
UseAAVTagsExpressionMammalianMutationD374YPromoterHCRApoE/hAATAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA
Plasmid#107307PurposeEncoding an SPH activation systemDepositorInsertCAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-F-FLEX- Kir2.1-T2A-tdTomato
Plasmid#60661PurposeFlp-dependent expression of Kir2.1-T2A-tdTomatoDepositorInsertFLEX- Kir2.1-T2A-tdTomato
UseAAVTagsExpressionMutationPromoterEF1αAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-D4H*-mCherry
Plasmid#134604Purposefluorescent cholesterol-binding probeDepositorInsertD4H*
UseTagsGST and mCherryExpressionBacterialMutationY415A and A463WPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
VCP(wt)-EGFP
Plasmid#23971DepositorInsertValosin containing protein (VCP Human)
UseTags6xHis, EGFP, and HAExpressionMammalianMutationnonePromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTRKH3-ermGFP
Plasmid#27169DepositorInserterythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
UseTagsExpressionBacterialMutationpresence of an additional 183-bp region at N term…PromoterAvailable sinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJR85
Plasmid#140095PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV Luciferase PGK-hygro
Plasmid#18782DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLuciferase and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pQE60-RRF-His
Plasmid#124132PurposeBacterial expression plasmid for PURE system componentsDepositorInsertRibosome recycling factor (frr Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only