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Plasmid#182095PurposeMammalian Expression of GFP scFv. Derived from hybridoma N86/38.DepositorInsertrecombinant mouse scFv targeting GFP
UseTagsHA, HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-eGFP
Plasmid#99375PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-eGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5E-elavl3
Plasmid#75025PurposeCan be used to drive pan neuronal expression in zebrafish.DepositorInsertELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
Use5' entry vector for multisite gateway three …TagsExpressionMutationPromoterelavl3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-iRFP670
Plasmid#99377PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-iRFP670
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NE1m
Plasmid#123308PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neuronsDepositorHas ServiceAAV Retrograde and AAV9InsertGPCR activation based NE sensor GRAB_NE1m
UseAAVTagsExpressionMutationcontains a glycine-to-threonine mutation at posit…PromoterhSynAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APPSwe-T2A-mCherry
Plasmid#190804PurposeExpress Swedish mutant APP from Ubiquitin C promoter, co-expressing mCherryDepositorInsertAPP (APP Human)
UseLentiviralTagsExpressionMutationSwedish (KM670/671NL)PromoterUbi CAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_NTS1.0
Plasmid#208670PurposeExpresses the genetically-encoded fluorescent neurotensin (NTS) sensor GRAB_NTS1.0 in mammalian cellsDepositorInsertGPCR activation based neurotensin (NTS) sensor GRAB_NTS1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_NPY1.0
Plasmid#208675PurposeExpresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in mammalian cellsDepositorInsertGPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH
Plasmid#203840PurposeKir2.1 expression for silencing neurons and mScarlet-KASH expressionDepositorInsertKir2.1 (Kcnj2 Mouse)
UseAAVTags2A-mScarlet-KASHExpressionMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_UCN1.0
Plasmid#208667PurposeExpresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in mammalian cellsDepositorInsertGPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2945
Plasmid#144421PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNEUROD1 (NEUROD1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
eMscL WT
Plasmid#107454PurposeeMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motifDepositorInsertbacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)
UseAdeno-associated viralTagsKir2.1 ER export signal (FCYENEV) and tdTomato fl…ExpressionMammalianMutationPromoterhuman synapsin 1Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_UCN1.0
Plasmid#208669PurposeExpresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in a cre-dependent mannerDepositorInsertGPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJEP316-pAAV-U6SaCas9gRNA(SapI)-pA Empty Cassette
Plasmid#113693PurposeU6 driven SaCas9 gRNA expression cassette without a gRNA. SapI can be used to clone in gRNAs.DepositorInsertSaCas9 gRNA Cassete
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE HA Chk2 KD, dominant negative chk2
Plasmid#41902DepositorInsertChk2 (CHEK2 Human)
UseRetroviralTagsHAExpressionMammalianMutationD347APromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDG462
Plasmid#100903PurposeSpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.DepositorInsertshumanized CRISPR associated protein 9 Nickase
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAG and T2A-PuroRExpressionMammalianMutationD10A mutant converts to NickasePromoterCBh and U6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV8-hSyn-flex-miR30-eGFP-shDyn
Plasmid#132716PurposeEncodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Pdyn gene, which encodes dynorphinDepositorInsertGFP-shDyn (Pdyn Rat)
UseAAVTagsExpressionMutationPromoterSynapsin 1Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-SOUL-P2A-TdTomato-WPRE-hGH-polyA
Plasmid#177576PurposeExpresses SOUL in neuronal cell typesDepositorInsertSOUL
UseAAVTagstdTomatoExpressionMutationPromoterhSynAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-GFAP-NLS-CasRx-NLS-FLAG
Plasmid#154000PurposeExpress CasRx for AAV packageDepositorInsertCasRx
UseCRISPRTagsFLAGExpressionMutationPromoterGFAPAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Vglut2 in situ probe
Plasmid#45639DepositorInsertVglut2 in situ probe (Slc17a6 Mouse)
UseIn situTagsExpressionMutationfragment contains nt 2477-2993 of slc17a6 (Vglut2…PromoterAvailable sinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOTTC590 - pCMV ER-iRFP
Plasmid#59136PurposeA plasmid that express endoplasmic reticulum-targeted iRFP under the CMV promoter.DepositorInsertER-localized Infrared Fluorescent Protein
UseTagsER retention signal and signal peptideExpressionMammalianMutationPromoterCMV-IEAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_NPYmut
Plasmid#208678PurposeExpresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in mammalian cellsDepositorInsertGPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-pur-CAG-Bi-DREADD
Plasmid#159457PurposeAAVS1 targeting donor plasmid with Bi-DREADD expression cassette and puromycin selection geneDepositorInsertBi-DREADD
UseTagsExpressionMammalianMutationfusion protein of hM3Dq-mCherry and HA-KORD seper…PromoterCAGAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
nAChR beta2 WT
Plasmid#24272DepositorInsertnAChR beta2 WT (Chrnb2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLV-CAG-F14F15S-Switch
Plasmid#99733PurposeFlp-based FLEX reporter (from RFP to GFP)DepositorInsertTagRFP and EGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE
Plasmid#118273PurposepAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoterDepositorInsertGCaMP6f
UseAAVTags6xHis (N terminal on insert), T7 epitope, and Xpr…ExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
nAChR alpha4 WT
Plasmid#24271DepositorInsertnAChR alpha4 WT (Chrna4 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMS-eGFP-RTP801
Plasmid#65057PurposeExpression of RTP801 in mammalian cellsDepositorInsertDDIT4 (Ddit4 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMV-IEAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJEP302-pAAV-CMV-dSaCas9-KRAB-pA
Plasmid#113677PurposeA CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.DepositorInsertde-catalyzed SaCas9
UseAAV and CRISPRTagsKRAB and NLSExpressionMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-F14F15S-splitTVA-EGFP-tTAv2
Plasmid#183352PurposeFlp-dependent helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene #100799)DepositorInsertTVA, EGFP and tTA
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP305-pAAV-EFS-dSaCas9-KRAB-pA
Plasmid#113681PurposeA EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.DepositorInsertde-catalyzed SaCas9
UseAAV and CRISPRTagsKRAB and NLSExpressionMutationPromoterAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)
Plasmid#159579PurposeE. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)DepositorInsertHis6-MBP-tev-Hs.NF1 DEF (1198-1530) (NF1 Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-NOS-IN133.3xFLAG.mCherry-WPRE
Plasmid#127868PurposepAAV plasmid expressing an NOS-IN133.3xFLAG.mCherry fusion protein under the hSyn promoterDepositorInsertNos1 (Nos1 Mouse)
UseAAVTags3xFLAG and mCherryExpressionMutationAmino acids 1-133 onlyPromoterhSynAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-hLMX1A-T2A-copGFP
Plasmid#170444PurposeLentiviral vector expressing human LMX1A (with silent mutation) with copGFP reporter gene, under control of EF1alpha promoter.DepositorInserthuman LMX1A
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJEP303-pAAV-CMV-dSaCas9-VP64-pA
Plasmid#113678PurposeA CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted regionDepositorInsertde-catalyzed SaCas9
UseAAV, CRISPR, and Synthetic BiologyTagsNLS and VP64ExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOTTC591 - pCMV Mem-iRFP
Plasmid#59134PurposeA plasmid that express membrane-targeted iRFP under the CMV promoter.DepositorInsertMembrane-localized Infrared Fluorescent Protein
UseTagsPalmitylation signalExpressionMammalianMutationPromoterCMV-IEAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOTTC592 - pCMV Mito-iRFP
Plasmid#59135PurposeA plasmid that express mitochondrial-targeted iRFP under the CMV promoter.DepositorInsertMitochondria-localized Infrared Fluorescent Protein
UseTagsMitochondria-targeting sequenceExpressionMammalianMutationPromoterCMV-IEAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
nAChR alpha4 YFP
Plasmid#15245DepositorInsertalpha4-YFP-M (Chrna4 Mouse)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
AAV-actin prom-dsRed-adra2a.1227.shRNA
Plasmid#67880Purposeexpresses dsRed and shRNA to knock down adra2a receptorsDepositorInsertdsRed and shRNA to knock down the mouse adra2 receptor
UseAAVTagsExpressionMammalianMutationPromoterchicken beta actinAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Paragranulin+linker 1
Plasmid#176915Purposeexpress Paragranulin with linker 1 in mammalian cellsDepositorInsertParagranulin + linker 1 (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgCOQ2
Plasmid#186025Purposeknock out COQ2 in mammalian cellsDepositorInsertCoenzyme Q2, Polyprenyltransferase (COQ2 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
R711-X12-566: His6-MBP-tev-Hs.NF1iso2 (1203-1530)
Plasmid#159580PurposeE. coli protein expression of His6-MBP-tev-Hs.NF1iso2 (1203-1530)DepositorInsertHis6-MBP-tev-Hs.NF1iso2 (1203-1530) (NF1 Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NFL(K363TAG)-FLAG
Plasmid#182656PurposeExpresses mouse neurofilament light chain with a TAG codon at the position K363 & C-terminal FLAG tag (DYKDDDDK) in mammalian cells. Can be used for amber codon suppression & click labeling of NFL.DepositorInsertmouse neurofilament light chain with a K363TAG mutation (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationK363TAGPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP304-pAAV-EFS-dSaCas9-VP64-pA
Plasmid#113679PurposeA EFS driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted regionDepositorInsertde-catalyzed SaCas9
UseAAV, CRISPR, and Synthetic BiologyTagsNLS and VP64ExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-CATULIN
Plasmid#118979PurposeExpresses full length of Catulin-GFP (Mus musculus). Contains CMV and T3 promoter for mammalian expression and mRNA production.DepositorInsertAlpha Catulin (Ctnnal1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
nAChR alpha4 CFP
Plasmid#15244DepositorInsertalpha4-CFP-M (Chrna4 Mouse)
UseTagsCFPExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
MSCV-TAP(FLAG-HA) CYLD
Plasmid#60031PurposeCYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintainedDepositorInsertCylindromatosis (CYLD Human)
UseRetroviralTagsFLAG, HA, and IRES PuroExpressionMammalianMutationPromoterLTR-driven expressionAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
b2AR->M71-IRES-tauGFP-ACNF TV
Plasmid#105198Purposetargeting vector to generate a replacement of CDS of M71 with CDS of mouse beta2-adrenergic receptor with expression of tauGFP.DepositorUseMouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-PHD2
Plasmid#223552PurposeLentivirus transfer plasmid for expression of full length human PHD2DepositorInsertPHD2 (EGLN1 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIG-LRRC37Bchimpanzee-HA
Plasmid#220794PurposeTo express the chimpanzee receptor LRRC37B in mammalian cells together with EGFPDepositorInsertLRRC37B
UseTagsHAExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only