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Plasmid#70696PurposeExpresses GST-tagged anti-mCherry Nanobody in E. coli cells.DepositorInsertmCherry-Nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSR43.6r
Plasmid#63197PurposeExpresses CcaS, Ho1, and PcyA constitutivelyDepositorInsertsCcaS
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR59.4
Plasmid#63175PurposeExpresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET24b-APEX2
Plasmid#111702PurposeFor IPTG-inducible expression of APEX2 in E. coliDepositorInsertAPEX2
UseTags6xHis and V5ExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
PSD-95-pTagRFP
Plasmid#52671Purposeexpression of TagRFP-tagged PSD-95DepositorInsertPSD-95 (Dlg4 Rat)
UseTagstagRFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-ADRβ2 Control Plasmid
Plasmid#101123PurposeExpression of SNAP-Tagged β2 Adrenergic Receptor in Mammalian cellsDepositorInsertBeta-2-Adrenergic Receptor (ADRB2 Human)
UseTagsSNAP-tag (SNAPf)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
mRFP-RBD(R59A)-mRFP
Plasmid#18664DepositorInsertRas binding domain of Raf
UseTagsmRFPExpressionMammalianMutationR59A mutation in RBDPromoterAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pF CAG luc hygro
Plasmid#67502PurposeConstitutive expression of firefly luciferase in mammalian cellsDepositorInsertFirefly luciferase
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
IKK-2 S177E S181E
Plasmid#11105PurposeMammalian expression of constitutively active human IKK-2DepositorInsertIKK-2 (IKBKB Human)
UseTagsFLAGExpressionMammalianMutationS177E and S181E, constitutively activePromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTP223
Plasmid#13263DepositorInsertPlac-gam-bet-exo
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pML107
Plasmid#67639PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformationDepositorInsertsCas9
single guide RNA expression cassette
UseCRISPRTagsExpressionYeastMutationPromoterpSNR52 and pTDH3 (aka GAP promoter)Available sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GINKO1
Plasmid#113112PurposeMammalian expression of genetically encoded green fluorescent potassium ion indicator GINKO1DepositorInsertGINKO1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
ptf-Galectin3
Plasmid#64149PurposeExpression in mammalian cellsDepositorInsertGalectin3 (LGALS3 Human)
UseTagsEGFP and mRFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-GFP-IRF3
Plasmid#127663PurposeOver-express EGFP-IRF3DepositorInsertIRF3 (IRF3 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-4
Plasmid#72679PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-p27
Plasmid#19937DepositorInsertCDKN1B cyclin-dependent kinase inhibitor 1B (CDKN1B Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
Opto-CRAC version 1
Plasmid#101245PurposeGenetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signalingDepositorInsertLOV2 and STIM1 cytosolic fragment
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
piRFP
Plasmid#31857Purposea phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imagingDepositorHas ServiceCloning Grade DNAInsertiRFP
UseTagsExpressionMammalianMutationT2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…PromoterCMVAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-IF2
Plasmid#124124PurposeBacterial expression plasmid for PURE system componentsDepositorInsertInitiation factor 2 (infB Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD33.1
Plasmid#36267DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPBADAvailable sinceOct. 19, 2017AvailabilityAcademic Institutions and Nonprofits only