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Plasmid#12247Purpose2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmidDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only
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pCAG-Flpe
Plasmid#13787PurposeMammalian expression of enhanced form of Flp recombinaseDepositorInsertFlpe
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
BE4-Gam
Plasmid#100806PurposeExpresses BE4-Gam in mammalian cellsDepositorInsertBE4-Gam
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNX32-Dest
Plasmid#105083PurposeYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloningDepositorTypeEmpty backboneUseTagsNubGExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-Tet1-CD
Plasmid#83341PurposeTo introduce MS2 coat protein fused Tet1-CDDepositorInsertMS2-Tet1-CD
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-gRNA-CBh-mCherry
Plasmid#91947PurposeExpression of empty gRNA cassette and mCherryDepositorInsertmCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/er-(n2)oxStayGold(c4)v2.0
Plasmid#186296PurposeA new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.DepositorInserter-(n2)oxStayGold(c4)v2.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGTR
Plasmid#63143PurposePCR template to construct polycistronic tRNA-gRNADepositorInsertgRNA-tRNA fusion
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLEM415-ldhL-mRFP1
Plasmid#99842PurposeExpression of RFP in LactobacillusDepositorInsertmonomeric red fluorescent protein
UseTagsFLAGExpressionBacterialMutationPromoterldhlAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCVhygro-F-G9a
Plasmid#41721PurposeRetroviral expression of human G9a in mammalian cellsDepositorInsertG9a (EHMT2 Human)
UseRetroviralTagsflagExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-human Dynamin 1aa K44A
Plasmid#22197DepositorInserthuman Dynamin 1aa K44A (DNM1 Human)
UseTagsGFPExpressionBacterial and MammalianMutationK44APromoterAvailable sinceNov. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRN11
Plasmid#84455Purposeshuttle plasmid for sarA P1-mCherry expressionDepositorInsertmCherry
UseShuttle vector e.coli-s.aureus, expression of sgf…TagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.GFP
Plasmid#57822Purpose3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter drivenDepositorInsertssgRNA
EFS
eGFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre
Plasmid#84561PurposepSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loopsDepositorInsertFLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
UseLentiviralTagsFlag, Suntag, oxBFP, AID (auxin-inducible degron)ExpressionMutationPromoterUbCAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
6His-MBP-TEV-huLbCpf1
Plasmid#90096PurposeBacterial expression plasmid for protein purificationDepositorInserthuLbCpf1
UseTags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialMutationPromoterAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+HyPer7-NES
Plasmid#136467PurposeMammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
UseTagsNuclear export signal from the HIV Rev proteinExpressionMammalianMutationPromoterCMV, SP6Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLII12Pglu-700uDelta6
Plasmid#17866DepositorInsertFLII12Pglu-700uDelta6
UseTagsCitrineExpressionMammalianMutation700uDelta6, ECFP insert in mglBPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-mMLH1dn
Plasmid#174825PurposeMammalian expression of mouse MLH1dn (codon optimized)DepositorInsertmouse MLH1dn (codon optimized)
UseTagsExpressionMammalianMutationdeletion of residues 758-760PromoterEF1aAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET MBP His6 LIC cloning vector (2Cc-T)
Plasmid#37237DepositorTypeEmpty backboneUseTagsHis6 and MBPExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe B-Raf V600E
Plasmid#17544DepositorInsertB-Raf (BRAF Human)
UseRetroviralTagsExpressionMammalianMutationV600EPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only