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AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

Plasmid

#65458

Purpose

Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

tTA2

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP

Plasmid

#139485

Purpose

PCR template for Halo-Tag gene knock-in donor vector

Depositor

Kazuhiro Aoki

Article

Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

Insert

HaloTag-loxP-P2A-dTKneo-pA-loxP

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pKS016 - pCAGGS-3XFLAG-mKate2-Pds5a-bpA

Plasmid

#156439

Purpose

For transient expression of mouse PDS5A tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse PDS5A (Pds5a Mouse)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75240

Purpose

CRISPR/Cas9 plasmid against human Ikaros

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Ikaros (IKZF1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200)

Plasmid

#119857

Purpose

Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200) (YTHDF1 100-200 subdomain)

Depositor

Bryan Dickinson

Article

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Insert

dCas13b-GGS-NYTHDF1(100-200)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579)

Plasmid

#133789

Purpose

CAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized CjeCas9

Use

Tags

NLS(SV40)-3xFLAG

Expression

Mammalian

Mutation

n/a

Promoter

CAG

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBW1604_pCAG-PV1-PYL-L1-PhiC31-234C-BGHpA

Plasmid

#108798

Purpose

Expresses aa234 to C-terminus of PhiC31 integrase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

PhiC31 integrase fragment aa234 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

Plasmid

#116955

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200)

Plasmid

#119856

Purpose

Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200) (YTDHF2 100-200 subdomain)

Depositor

Bryan Dickinson

Article

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Insert

dCas13b-GGS-NYTHDF2(100-200)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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Ikaros-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75241

Purpose

CRISPR/Cas9 plasmid against human Ikaros

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Ikaros (IKZF1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pKS023 - pCAGGS-3XFLAG-mKate2-Pds5b-bpA

Plasmid

#156442

Purpose

For transient expression of mouse PDS5B tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse PDS5B (Pds5b Mouse)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCasper4-tubulin-IVS-Syn21-QSco-p10

Plasmid

#46132

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QSco

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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p23-NES-PspCas13b-msfGFP-NES-Flag

Plasmid

#165071

Purpose

overexpression of PspCas13b in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

PspCas13b

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP

Plasmid

#139484

Purpose

PCR template for mEGFP gene knock-in donor vector

Depositor

Kazuhiro Aoki

Article

Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

Insert

mEGFP-loxP-P2A-dTKneo-pA-loxP

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

Plasmid

#114089

Purpose

CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

Depositor

Benjamin Kleinstiver, Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized FnCas12a

Use

Tags

NLS(nucleoplasmin) and 3xHA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG APX W41F dimeric (mito matrix)

Plasmid

#40307

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

pea APX W41F (dimeric)

Use

Tags

Flag

Expression

Mutation

W41F mutation.

Promoter

CAG

Available since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-hFIP200(1363-1370Mut)-MBP

Plasmid

#190038

Purpose

Expression of FIP200 mutant

Depositor

James Hurley

Article

Shi et al Elife. 2020 Aug 10;9. pii: 59099. doi: 1

Insert

FIP200 (RB1CC1 Human)

Use

Tags

GST and MBP

Expression

Mammalian

Mutation

1363 RDKDLIES 1370 to GSSGGSSG

Promoter

CMV

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)

Plasmid

#134331

Purpose

CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized Nme2Cas9

Use

Tags

NLS(SV40)-3xFLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA

Plasmid

#156441

Purpose

For transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse SA2 (Stag2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pBW808_pCAG-PV5-VloxP-loxN-F3-GFP-BGHpA

Plasmid

#87584

Purpose

PV5 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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