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Plasmid#68132PurposeExpression of Cerulean-tagged Plk1 in mammalian cellsDepositorInsertPolo-like kinase 1 (PLK1 Human)
UseTagsCeruleanExpressionMammalianMutationwild-typePromoterCMVAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACYC-GST
Plasmid#62329PurposeE. coli pACYC based expression vector with N-terminal TEV cleavable GST-tagDepositorTypeEmpty backboneUseTagsGlutathione S-transferase (GST) tag and TEV prote…ExpressionBacterialMutationPromoterT7-lac0Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pChillyBoys_gen1
Plasmid#228495PurposepACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)DepositorInsertsgroEL
groES
UseTagsExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHL1726
Plasmid#53032PurposePLtetO-1:hfqlead::mCherry, PLlacO-1:RBS(st7):csrADepositorInsertsmCherry
csrA
UseSynthetic BiologyTagsRBS(st7) and hfqleadExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pExp-DsbC
Plasmid#112564PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-DsbC and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-BG4-NLS-mCer
Plasmid#211448Purposemammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequenceDepositorInsertAnti-DNA G-quadruplex single chain antibody BG4
UseTagsNLS-mCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
tetO-GFPnls-mPGK-rtTA-IRES-Puro
Plasmid#69545PurposeTet-On system expressing GFP tagged with 2x nuclear localization signals.DepositorInserteGFP
UseLentiviralTags2x nuclear localization sequence and T2A "sk…ExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pExp-DsbA(SS)
Plasmid#112563PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-DsbA(SS) and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
N1-LOC8R
Plasmid#66896PurposeLeader Of Cox8 Repeated (LOC8R) is a synthetic mitochondrial targeting sequence compatible with any N1 vector. Internal restriction sites enable modular subcloning of 1x, 2x, 3x or 4x presequences.DepositorInsertLOC8R
UseTagsExpressionMutationPromoterAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLIC
Plasmid#27989DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHL1746
Plasmid#53034PurposePLtetO-1:ycdTlead::mCherry, PLlacO-1:RBS(st7):csrADepositorInsertsmCherry
csrA
UseSynthetic BiologyTagsRBS(st7) and ycdTleadExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
WT_H1-S1
Plasmid#218400Purpose3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in first intronDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT_S1-H1
Plasmid#218392Purpose3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in second intronDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-N
Plasmid#218398Purpose3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_N-H1
Plasmid#218397Purpose3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT_S7-H1
Plasmid#218393Purpose3-exon minigene, with SRSF7 motif-rich middle exon, and hnRNPA1 motif-rich region in second intronDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE80l B-CC-B
Plasmid#196479PurposeProtein expression of SpyCatcher-ELP-CarHCC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCCDepositorInsertB-CC-B
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJK636
Plasmid#72466PurposeProduces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL1747
Plasmid#53035PurposePLtetO-1:ydeHlead::mCherry, PLlacO-1:RBS(st7):csrADepositorInsertsmCherry
csrA
UseSynthetic BiologyTagsRBS(st7) and ydeHleadExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only