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Addgene
Showing: 2161 - 2180 of 13889 results

  1. MUT_S1-spacer-H1

    Plasmid
    #218399
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Availability
    Academic Institutions and Nonprofits only
    218399-plasmid-map-sequence-id-436404

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  2. G-SP140-8

    Plasmid
    #166520
    Purpose
    scFv of a human scaffold targeting Nuclear body protein SP140. Antigen coverage aa 688-862 of 867, Bromodomain
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Availability
    Academic Institutions and Nonprofits only
    166520-plasmid-map-sequence-id-329134

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  3. Venus-30aa-Noxa-pEGFP-C1

    Plasmid
    #166762
    Purpose
    To increase the length of the linker between Venus and Noxa proteins
    Depositor
    David Andrews
    Insert
    Noxa (PMAIP1 Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    166762-plasmid-map-sequence-id-328844

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  4. Venus-30aa-Noxa-4E-pEGFP-C1

    Plasmid
    #167529
    Purpose
    To increase the length of the linker between Venus and Noxa-4E proteins
    Depositor
    David Andrews
    Insert
    PMA-induced protein 1 (PMAIP1 Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    C25E, L29E, F32E, L36E
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    167529-plasmid-map-sequence-id-347649

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  5. pAAV-cFOS-FRB-VP16-P2A-EGFP

    Plasmid
    #210510
    Purpose
    expresses cFOS-FRB-VP16 and EGFP component in rat hippocampal neuron cells
    Depositor
    Dongmin Lee
    Insert
    FRB-VP16-P2A-EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    cFos
    Availability
    Academic Institutions and Nonprofits only
    210510-plasmid-map-sequence-id-415565

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  6. DRD2-V2tail-TevN-BLITz-TetR-FKBP

    Plasmid
    #210514
    Purpose
    expresses DRD2-V2tail-TevN-BLITz-TetR-FKBP component in mammalian cells
    Depositor
    Dongmin Lee
    Insert
    DRD2-V2tail-TevN-BLITz-TetR-FKBP
    Use
    Tags
    HA signal FALG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    210514-plasmid-map-sequence-id-415568

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  7. pT7CFE1_polio_3ABC

    Plasmid
    #212986
    Purpose
    Encodes Poliovirus type I Mahoney 3ABC optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney 3ABC
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    212986-plasmid-map-sequence-id-420355

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  8. pT7CFE1_polio_VP12A

    Plasmid
    #212988
    Purpose
    Encodes Poliovirus type I Mahoney VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus type I Mahoney VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Expression
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    212988-plasmid-map-sequence-id-420361

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  12. TALEN_C18

    Plasmid
    #47974
    Purpose
    Golden Gate Compatible TALEN Construct site specific modification of genome, shortened C Terminus, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Shortened C-Terminus,Codon Optimized FokI Endonuc…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    47974-plasmid-map-sequence-id-68905

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  13. pET-HIS-Sangamo

    Plasmid
    #40786
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    N Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    40786-plasmid-map-sequence-id-68901

    Enlarge

  14. pET-Sangamo-HIS

    Plasmid
    #40787
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    C Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus,Codon Optimized FokI Endonuclease
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    40787-plasmid-map-sequence-id-68902

    Enlarge

  16. pCeru-N1 cyclin A (1557)

    Plasmid
    #61842
    Purpose
    mammalian expression of cyclin A with cerulean fusion
    Depositor
    Jonathon Pines
    Insert
    cyclin A (CCNA2 Human)
    Use
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
Showing: 2161 - 2180 of 13889 results