-
Plasmid#15820DepositorInsertCullin3 (CUL3 Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N418PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
MS2-P65-HSF1_GFP
Plasmid#61423PurposeExpresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 3rd generation lentiviral vector.DepositorInsertMS2(N55K)-P65-HSF1_2A_GFP (HSF1 Synthetic, Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP II (pMIG II)
Plasmid#52107Purposea bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsIRES-EGFPExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1
Plasmid#72266PurposeExpress gene of interest under EF-1 promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3
Plasmid#171588PurposeLentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoterDepositorInsertsBxb1 integrase
iCasp9
BFP
rtTA3G
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-i_Giantin_C1
Plasmid#85050PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorInsertGiantin (GOLGB1 Human)
UseTagsExpressionMammalianMutationaa 3131-3259PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
WGA-D
Plasmid#17989DepositorInsertwheat germ agglutinin isolectin D
UseTagsExpressionBacterialMutationN/APromoterAvailable sinceJuly 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ptet-RFP-shR-rtTA
Plasmid#35625DepositorTypeEmpty backboneUseAAV and RNAi; Tet inducibleTagsExpressionMammalianMutationPromoterPtetAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
DsRed2-pBAD
Plasmid#54608PurposeLocalization: Protein Expression Vector , Excitation: 558, Emission: 583DepositorTypeEmpty backboneUseTags6xHis and DsRed2ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
Flag-HA-COPS6
Plasmid#22542DepositorInsertCOPS6 (COPS6 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceJune 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
lenti-EF1a-dCas9-VPR-Puro
Plasmid#99373Purpose3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)DepositorInsert(Sp)dCas9-VPR-P2A-Puro
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV_NLS-dSaCas9-NLS-VPR
Plasmid#68495PurposeAAV vector containing nuclease null SaCas9 fused to VPRDepositorInsertdSaCas9
UseAAVTagsVPRExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCWX-UBI-hTert-PGK-BSD
Plasmid#114316PurposeConstitutive expression of the hTert proteinDepositorInserthTERT (TERT Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-G-GECO1.2
Plasmid#32446PurposeMammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)DepositorArticleInsertG-GECO1.2
UseTagsExpressionMammalianMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/hChR2(H134R)-mCherry
Plasmid#20938PurposeMammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activationDepositorInsertchannelrhodopsin-2
UseTagsExpressionMammalianMutationH134R mutation in ChR2PromoterCMVAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
c-Flag pcDNA3
Plasmid#20011PurposeThis pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
R777-E283 Hs.ROCK1
Plasmid#70567PurposeGateway ORF clone of human ROCK1 [NM_005406.2] with stop codon (for native or N-terminal fusions)DepositorInsertROCK1 (ROCK1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX301
Plasmid#25895DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSLTS
Plasmid#59386PurposeExpresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.DepositorTypeEmpty backboneUseGenome engineeringTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLSSmOrange-C1
Plasmid#37131DepositorInsertLSSmOrange
UseTagsExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable sinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits only