We narrowed to 15,811 results for: LEA

1. pFUW-TetO-tdTomato-cNLS

   Plasmid

   #138532

   Purpose

   Lentiviral inducible expression of tdTomato with nuclear localization signal

   Depositor

   Christopher Grigsby, Ana Teixeira

   Article

   Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

   Insert

   tdTomato
   

   Use

   Lentiviral

   Tags

   3x SV40 cNLS

   Expression

   Mammalian

   Promoter

   TRE-CMV

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. TfR-mCh-DHFR

   Plasmid

   #173013

   Purpose

   For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter.

   Depositor

   Matthew Kennedy

   Article

   Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   TfR-mCh-DHFR
   

   Tags

   DHFR and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCVL.Active/Repressed TLR (Sce)

   Plasmid

   #45575

   Purpose

   Codes for the Active/Repressed TLR with the target site for I-Sce I nuclease

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   eGFP with I-Sce I TS
   +3 mCherry
   

   Use

   Lentiviral

   Tags

   T2A dislinker and iRFP

   Expression

   Bacterial and Mammalian

   Mutation

   embedded I-Sce I TS from 163-185

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. RHBDD1 pmRFP-N2 (1390)

   Plasmid

   #62049

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   RHBDD1 (RHBDD1 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

5. PB_EF1a-CasRx-msfGFP-2A-Blast

   Plasmid

   #226010

   Purpose

   CasRx protein for RNA targeting in piggyBac backbone

   Depositor

   Silvana Konermann

   Article

   Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

   Insert

   CasRx RNA-targeting nuclease
   

   Use

   CRISPR

   Tags

   msfGFP

   Expression

   Mammalian

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUW-TetO-mCherry-cNLS

   Plasmid

   #138531

   Purpose

   Lentiviral inducible expression of mCherry with nuclear localization signal

   Depositor

   Christopher Grigsby, Ana Teixeira

   Article

   Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   3x SV40 cNLS

   Expression

   Mammalian

   Promoter

   TRE-CMV

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RHBDD1 pEGFP-N2 (1078)

   Plasmid

   #62028

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   RHBDD1 (RHBDD1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTriEx-mCherry-LINXa3

   Plasmid

   #73614

   Purpose

   Light-induced nuclear export switch for microscopy in mammalian cells.

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

   Insert

   LINXa3
   

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   Chicken B-Actin

   Available Since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. DHFR-GFP-th-NL1

   Plasmid

   #173007

   Purpose

   For light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.

   Depositor

   Matthew Kennedy

   Article

   Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   DHFR-GFP-NL1
   

   Tags

   DHFR and EGFP

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-AMPKsub-YPet-NES

    Plasmid

    #84635

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub-YPet-NES
    

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. DHFR-GFP-th-GluA1

    Plasmid

    #173008

    Purpose

    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    DHFR-GFP-th-GluA1
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-REPORT(PGK)

    Plasmid

    #172328

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTriEx-mCherry-LINXa3-nano

    Plasmid

    #73616

    Purpose

    Light-induced nuclear export switch fusion to nano for microscopy in mammalian cells.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    LINXa3-nano
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUW-TetO-DsRed-cNLS

    Plasmid

    #138533

    Purpose

    Lentiviral inducible expression of DsRed-Express2 with nuclear localization signal

    Depositor

    Christopher Grigsby, Ana Teixeira

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    DsRed-Express2
    

    Use

    Lentiviral

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Promoter

    TRE-CMV

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Src(WT)-SsrA(Y7I)-mCer3

    Plasmid

    #220998

    Purpose

    This is microTag (Y7I mutation in SsrA)-Src with mCerulean3 at C-terminal

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    Src, SsrA, mCerulean3 (Src Mouse)
    

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Y7I in SsrA

    Promoter

    CMV

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. P9_TEVs_cLuc

    Plasmid

    #119303

    Purpose

    Expresses C-terminus of split luciferase connected to P9 coil via TEVs cleavable linker/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cLuc connected to P9 coil via TEVs cleavable linker
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-mCherry-TSC2(AA)

    Plasmid

    #192442

    Purpose

    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2(S939A,T1439A) (TSC2 Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. WFS1 pEGFP-N2 (1472)

    Plasmid

    #62051

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    WFS1 (WFS1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pAFMW-Nbr-D435A/E437A

    Plasmid

    #40829

    Purpose

    Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

    Depositor

    Phillip Zamore

    Article

    Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

    Insert

    Nbr D435A/E437A (Nbr Fly)
    

    Tags

    3xFLAG and 6xMyc

    Expression

    Insect

    Mutation

    D435A/E437A, part of the DEDD motif; mutations p…

    Promoter

    Actin5C

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

20. POPDC2 pEGFP-N2 (1087)

    Plasmid

    #62030

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    POPDC2 (POPDC2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only