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pINDUCER20-FLAG-hMYOD1 (#800)

Plasmid

#78330

Purpose

Doxycycline-inducible lentiviral overexpression of human MYOD1 ORF with a FLAG tag

Depositor

Louis Kunkel, Matthew Alexander

Insert

MYOD1 (MYOD1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

TRE2

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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CRY2-OCRL

Plasmid

#79566

Purpose

expression of CRY2PHR fused to inositol 5-phosphatase domain of OCRL

Depositor

Olof Idevall-Hagren

Insert

OCRL (OCRL Human)

Use

Tags

CRY2 (photolyase homology region domain of Arabid…

Expression

Mammalian

Mutation

aa 234_539

Promoter

CMV

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only
mTurquoise2-Rab7(WT)

Plasmid

#112959

Purpose

In vivo visualization of Rab7 (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

Rab7

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc12-HiBiT

Plasmid

#198308

Purpose

Produces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assays

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc12-HiBiT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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6His-MBP-TEV-huAsCpf1

Plasmid

#90095

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huAsCpf1

Use

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Mutation

Promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-VARNAM2-Kv2.1PR

Plasmid

#195530

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Tags

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CaMKII

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pJM253 (miniCTX1-rhaSR-PrhaBAD)

Plasmid

#110571

Purpose

Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhaSR-PrhaBAD

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-ABEmax-BlastR

Plasmid

#152989

Purpose

A-to-G base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

ABE7.10-nSpCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro-SOX2

Plasmid

#47540

Purpose

Tet inducible knockdown of SOX2.

Depositor

Charles Rudin

Article

Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

Insert

SOX2 shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pGG-TurboID

Plasmid

#209391

Purpose

Transiently expressing TurboID in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

3XHA-TurboID

Use

Tags

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6d-V106W

Plasmid

#215827

Purpose

Express CBE6d V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6d V106W-SpCas9-2xUGI (cas9 )

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pTK204

Plasmid

#13534

Depositor

Wolf Frommer

Article

Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

Insert

FLIPW-CTYT

Use

Tags

See map

Expression

Bacterial

Mutation

Promoter

Available since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

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pRK5 TGF beta type I receptor Flag

Plasmid

#14831

Depositor

Rik Derynck

Article

Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

Insert

TGF beta type I receptor (Tgfbr1 Rat)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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pBAD-6xHis-AviTag-ALFAtag-eGFP

Plasmid

#195854

Purpose

Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

6xHis-AviTag-ALFAtag-eGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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SuExp His-Myc-mPLD4

Plasmid

#201185

Purpose

Protein expression plasmid for recombinant His-Myc tag mouse PLD4

Depositor

David Nemazee

Article

Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

Insert

PLD4 (Pld4, Pld4, Pld4 Mouse)

Use

Tags

His-Myc, Factor Xa cleavable

Expression

Mammalian

Mutation

cytosolic and transmembrane domain truncated; add…

Promoter

CMV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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iRFP-C1

Plasmid

#54786

Purpose

Localization: C1 Cloning Vector, Excitation: 690, Emission: 713

Depositor

Vladislav Verkhusha, Michael Davidson

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Type

Empty backbone

Use

Tags

iRFP

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pPSU30

Plasmid

#173830

Purpose

Expresses 30 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

HSTPABPACS100BCBP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPSU10

Plasmid

#173827

Purpose

Expresses 10 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPAB

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBT376

Plasmid

#125617

Purpose

Expresses BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

BE4max-NG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-rDA3m

Plasmid

#208702

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA3m

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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