160,877 results

1. LentiGuide Puro-P2A-EGFP

   Plasmid

   #137729

   Purpose

   For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).

   Depositor

   Fredrik Wermeling

   Article

   Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPBbsr-AcGFP1-ORP9-PH

   Plasmid

   #214270

   Purpose

   Mammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PH

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   AcGFP1-ORP9(1-111) (Osbpl9 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCasPA

   Plasmid

   #113347

   Purpose

   Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

   Depositor

   Quanjiang Ji

   Article

   Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-hCdt1-HA

   Plasmid

   #117500

   Purpose

   Expresses human Cdt1 fused to HA tag

   Depositor

   Domenico Maiorano

   Article

   Tsanov et al Nucleic Acids Res. 2014 Apr;42(6):3692-7

   Insert

   Cdc10-dependent transcript-1 (CDT1 Human, 1700 bp)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CMV

   Available since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRK5 FLAG metap2

   Plasmid

   #32004

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2011 Aug 5;146(3):408-20.

   Insert

   metap2 (Metap2 )
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

6. 2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH

   Plasmid

   #185713

   Purpose

   Recombinant B. mori truncated R2 RT expression

   Depositor

   Kathleen Collins

   Article

   Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

   Insert

   B. mori truncated R2 RT
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Changed W403 and F753 to A

   Promoter

   T7

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMSP1

   Plasmid

   #20060

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

   Insert

   MSP1 (APOA1 synthetic gene)
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   synthetic gene of deletion mutant (1-43) of human…

   Promoter

   Available since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-C-terminal GST

   Plasmid

   #128025

   Purpose

   Mammalian expression vector with C terminal GST tag

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSANG10-3F-BG4

   Plasmid

   #55756

   Purpose

   Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

   Depositor

   Shankar Balasubramanian

   Article

   Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

   Insert

   BG4
   

   Use

   Tags

   6xHis, pelB leader, and tri-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBS L30 mRuby3-Rab6b

    Plasmid

    #166427

    Purpose

    Rab5C fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Insert

    Rab6B
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    L30 weak promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPath-Violacein-pT7native

    Plasmid

    #200002

    Purpose

    Violacein pathway. pT7-native CDS sequence

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Pathways
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. lenti F9-TetR-EGFP-IRES-PuroR

    Plasmid

    #117049

    Purpose

    lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Insert

    F9-TetR-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    F9 promoter

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MSCV-human Igf2bp2-IRES-GFP

    Plasmid

    #91890

    Purpose

    Retroviral expression of human Igfbp2

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    Igf2bp2 (IGF2BP2 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FLAG-NLRC5

    Plasmid

    #37521

    Depositor

    Thomas Kufer

    Article

    Neerincx et al J Biol Chem. 2010 Aug 20;285(34):26223-3

    Insert

    NLRC5 (NLRC5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV-EF1A-CD19.8h.8TM.28.3z

    Plasmid

    #200670

    Purpose

    Modular CD8hinge-CD8transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD8TM-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFUGW mCherry-KASH

    Plasmid

    #131505

    Purpose

    mCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-in

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    mCherry_KASH
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUBC

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYLCRISPR/Cas9Pubi-B

    Plasmid

    #66188

    Purpose

    expression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    maize ubiquitin promoter (Pubi)

    Available since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSCV-mLin28A

    Plasmid

    #26357

    Depositor

    George Daley

    Article

    Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

    Insert

    lin-28 homolog A (Lin28a Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pSTARR-seq_human

    Plasmid

    #71509

    Purpose

    humanSTARR-seq screening vector with SCP1 core promoter

    Depositor

    Alexander Stark

    Article

    Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

    Type

    Empty backbone

    Use

    Humanstarr-seq screening vector

    Tags

    sgGFP (SuperGlo, Qbiogene, Inc)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

20. LiOn-CAG∞RFP

    Plasmid

    #154016

    Purpose

    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

    Depositor

    Jean Livet

    Article

    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

    Insert

    mRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H210P

    Promoter

    CAG

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge