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Addgene
Showing: 2081 - 2100 of 13889 results
  1. pHL1790

    Plasmid
    #53038
    Purpose
    PLtetO-1:glgClead::mCherry, PLlacO-1:csrB
    Depositor
    Inserts
    mCherry
    csrB
    Use
    Synthetic Biology
    Tags
    glgC leader
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Availability
    Academic Institutions and Nonprofits only
  2. pACYC-GST

    Plasmid
    #62329
    Purpose
    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Glutathione S-transferase (GST) tag and TEV prote…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lac0
    Availability
    Academic Institutions and Nonprofits only
  3. pCer-C3-Plk1

    Plasmid
    #68132
    Purpose
    Expression of Cerulean-tagged Plk1 in mammalian cells
    Depositor
    Insert
    Polo-like kinase 1 (PLK1 Human)
    Use
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  4. pCMV-NLS-FRB-VP16

    Plasmid
    #210515
    Purpose
    expresses NLS-FRB-VP16 component in mammalian cells
    Depositor
    Insert
    NLS-FRB-VP16
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3-RPA32-mCer

    Plasmid
    #211387
    Purpose
    mammalian expression of human RPA32 C-terminally fused to mCerulean
    Depositor
    Insert
    RPA32 (RPA2 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pHL1722

    Plasmid
    #53030
    Purpose
    PLtetO-1:glgClead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and glgC leader
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Availability
    Academic Institutions and Nonprofits only
  7. pHL1724

    Plasmid
    #53031
    Purpose
    PLtetO-1:pgaAleadFull::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and pgaAleadFull
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Availability
    Academic Institutions and Nonprofits only
  8. pHL1745

    Plasmid
    #53033
    Purpose
    PLtetO-1:cstAlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and cstAlead
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Availability
    Academic Institutions and Nonprofits only
  9. pHL1746

    Plasmid
    #53034
    Purpose
    PLtetO-1:ycdTlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and ycdTlead
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Availability
    Academic Institutions and Nonprofits only
  10. pHL1747

    Plasmid
    #53035
    Purpose
    PLtetO-1:ydeHlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and ydeHlead
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Availability
    Academic Institutions and Nonprofits only
  11. pHL1914

    Plasmid
    #53042
    Purpose
    PconNoHindM2:hfqlead::gfp, PconNoHind:RBS(st3):tetR
    Depositor
    Inserts
    GFP
    tetR
    Use
    Synthetic Biology
    Tags
    RBS(st3) and hfqlead
    Expression
    Bacterial
    Mutation
    Promoter
    PconNoHindM2 and pLlacO-1
    Availability
    Academic Institutions and Nonprofits only
  12. N1-LOC8R

    Plasmid
    #66896
    Purpose
    Leader Of Cox8 Repeated (LOC8R) is a synthetic mitochondrial targeting sequence compatible with any N1 vector. Internal restriction sites enable modular subcloning of 1x, 2x, 3x or 4x presequences.
    Depositor
    Insert
    LOC8R
    Use
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  13. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69545
    Purpose
    Tet-On system expressing GFP tagged with 2x nuclear localization signals.
    Depositor
    Insert
    eGFP
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  14. pJK636

    Plasmid
    #72466
    Purpose
    Produces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)
    Depositor
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  15. pExp-DsbA(SS)

    Plasmid
    #112563
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Type
    Empty backbone
    Use
    Tags
    8xHis-DsbA(SS) and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  16. pExp-DsbC

    Plasmid
    #112564
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Type
    Empty backbone
    Use
    Tags
    8xHis-DsbC and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  17. WT_S1-H1

    Plasmid
    #218392
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron
    Depositor
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Availability
    Academic Institutions and Nonprofits only
  18. WT_S7-H1

    Plasmid
    #218393
    Purpose
    3-exon minigene, with SRSF7 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron
    Depositor
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Availability
    Academic Institutions and Nonprofits only
  19. MUT_N-H1

    Plasmid
    #218397
    Purpose
    3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 2081 - 2100 of 13889 results