We narrowed to 15,836 results for: LEA

1. NET5L pEGFP-N2 (1155)

   Plasmid

   #62035

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   NET5L (TMEM201 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   long form

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pLV-REPORT(EF1a)-TTN

   Plasmid

   #172327

   Purpose

   LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   d2eGFP
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cp PS Intein dCas9-VPR

   Plasmid

   #242031

   Purpose

   Blue-light-controlled release of dCas9-VPR from the cytoplasm to the nucleus.

   Depositor

   Vladislav Verkhusha

   Article

   Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

   Insert

   Cp PS Intein dCas9-VPR
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTE4398

   Plasmid

   #74042

   Purpose

   Expresses human codon-optimized LbCpf1 and Lb crRNA.

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   Lb crRNA
   LbCpf1
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Promoter

   CMV and human U6

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pExpreS2-1-C3-10H-SIII

   Plasmid

   #175444

   Purpose

   HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 Platform

   Depositor

   Wyatt Yue

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Tags

   3C-10His-TwinStrep and BiP Secretion Peptide

   Expression

   Insect

   Promoter

   Fused Actin-HSP70

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

   Plasmid

   #117687

   Purpose

   U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

   Depositor

   Erik Sontheimer

   Article

   Gao et al Methods Enzymol. 2019;616:365-383. doi:

   Inserts

   Kanamycin cassette
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and hPGK

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS CMV GAG

   Plasmid

   #234992

   Purpose

   For production of Viral like particles (VLPs) by expressing the MLV GAG protein in mammalian cells

   Depositor

   Monica Gonzalez-Magaldi, Daniel Leahy

   Article

   Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

   Insert

   GAG
   

   Expression

   Mammalian

   Mutation

   Deleted polymerase coding sequence

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDuBir-Lbu-dCas13a-avitag

   Plasmid

   #100817

   Purpose

   Dual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13a

   Depositor

   Mitchell O'Connell

   Article

   Tambe et al Cell Rep. 2018 Jul 24;24(4):1025-1036. d

   Insert

   Lbu_Cas13a_R472A_H477A_R1048A_H1053A
   

   Tags

   AviTag and His6-MBP

   Expression

   Bacterial

   Mutation

   R472A, H477A, R1048A, H1053A

   Promoter

   T7

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Nuc-ExRai-AktAR2

   Plasmid

   #184049

   Purpose

   Nuclear-targeted ExRai-AktAR2.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   Nuc-ExRai-AktAR2
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTE4396

    Plasmid

    #74041

    Purpose

    Expresses human codon-optimized AsCpf1 and As crRNA.

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    As crRNA
    AsCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Promoter

    CMV and human U6

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pExpreS2-1-C3-PA-10H-SIII

    Plasmid

    #175443

    Purpose

    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Tags

    3C-Protein A-10His-TwinStrep and BiP Secretion Pe…

    Expression

    Insect

    Promoter

    Fused Actin-HSP70

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-BirA-P2A-mCherry

    Plasmid

    #74056

    Purpose

    retroviral expression plasmid for selfcleaving E.coli biotin ligase BirA-P2A-mCherry fusion protein

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    BirA biotin ligase
    

    Use

    Retroviral

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Promoter

    MSCV-LTRs

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGT9050

    Plasmid

    #219429

    Purpose

    PapE Exonuclease fused to linker 4LF2 as N-terminal tag module (AATG_PapE-4*LF2_AGGT)

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    AATG_PapE-4xLF2_AGGT
    

    Use

    Moclo compatible level 0 module

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-EF1a-EGFP-Emerin-IRES-Hygromycin

    Plasmid

    #134864

    Purpose

    Expresses EGFP-tagged nuclear envelope marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    emerin (EMD Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tmem38A pEGFP-N2 (1401)

    Plasmid

    #62050

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    Tmem38A (TMEM38A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pNLS-2xBFP(4C)

    Plasmid

    #176151

    Purpose

    Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    NLS-2xBFP(4C)
    

    Tags

    6xHis tag and SV40 nuclear localization sequence …

    Expression

    Bacterial

    Mutation

    Compared with EGFP, the two BFP domains have the …

    Promoter

    Ptrc

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tmem38A mRFP-N2 (800)

    Plasmid

    #62007

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    Tmem38A (TMEM38A Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pExodus CMV.Trex2

    Plasmid

    #40210

    Purpose

    Mammalian expression of codon-optimized Trex2.

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

    Insert

    Three prime repair exonuclease 2 (Trex2 Mouse)
    

    Expression

    Bacterial and Mammalian

    Promoter

    CMV and T7

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Akt-STOPS-NLS

    Plasmid

    #175007

    Purpose

    Nuclear-targeted Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-STOPS-NLS
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. SWEET11:SWEET11-2A-GFP-GUS

    Plasmid

    #172484

    Purpose

    Translational fusion of SWEET11 with a P2A self cleaving peptide sequence

    Depositor

    Wolf Frommer

    Article

    Kim et al Plant Cell. 2021 May 5;33(3):511-530. do

    Insert

    SWEET11 (AT3G48740 Mustard Weed)
    

    Tags

    GFP, GUS

    Expression

    Plant

    Promoter

    SWEET11

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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