We narrowed to 15,678 results for: LEA

1. pTriEx-mCherry-LINXa3-nano

   Plasmid

   #73616

   Purpose

   Light-induced nuclear export switch fusion to nano for microscopy in mammalian cells.

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

   Insert

   LINXa3-nano
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. P9_TEVs_cLuc

   Plasmid

   #119303

   Purpose

   Expresses C-terminus of split luciferase connected to P9 coil via TEVs cleavable linker/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   split cLuc connected to P9 coil via TEVs cleavable linker
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-mCherry-TSC2(AA)

   Plasmid

   #192442

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A) (TSC2 Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. WFS1 pEGFP-N2 (1472)

   Plasmid

   #62051

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   WFS1 (WFS1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pAFMW-Nbr-D435A/E437A

   Plasmid

   #40829

   Purpose

   Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

   Depositor

   Phillip Zamore

   Article

   Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

   Insert

   Nbr D435A/E437A (Nbr Fly)
   

   Use

   Tags

   3xFLAG and 6xMyc

   Expression

   Insect

   Mutation

   D435A/E437A, part of the DEDD motif; mutations p…

   Promoter

   Actin5C

   Available Since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

6. NET5L pEGFP-N2 (1155)

   Plasmid

   #62035

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   NET5L (TMEM201 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   long form

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

7. POPDC2 pEGFP-N2 (1087)

   Plasmid

   #62030

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   POPDC2 (POPDC2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

8. NKP68 RFP (790)

   Plasmid

   #62003

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

   Insert

   NKP68 (TMUB1 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   pgk

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

9. NET30 pEGFP-N2 (815)

   Plasmid

   #62011

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   NET30 (MOSPD3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

10. CKAP2-mNeonGreen pHAT

    Plasmid

    #175947

    Purpose

    Bacterial expression of mmCKAP2-mNeonGreen, N-terminal 6xHis-tag, C-terminal Strep-tagII

    Depositor

    Susanne Bechstedt

    Article

    McAlear et al Elife. 2022 Jan 14;11. pii: 72202. doi:

    Insert

    CKAP2 (Ckap2 Mouse)
    

    Use

    Tags

    6His, StrepTagII, and mNeonGreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-REPORT(EF1a)-TTN

    Plasmid

    #172327

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. H2B-mScarlet- hRec8(297-506)-hMeikin(1-332, E151R, R154E)- mNeonGreen

    Plasmid

    #174716

    Purpose

    Stable expression of fluorescent Separase cleavage sensor with human Rec8 fused to human Meikin

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Rec8 (REC8 Human)
    

    Use

    Retroviral

    Tags

    H2B-mScarlet and mNeonGreen

    Expression

    Mammalian

    Mutation

    hRec8: 297-506; hMeikin: 1-332, E151R, R154E

    Promoter

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. NET55 pEGFP-N2 (610)

    Plasmid

    #61992

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET55 (APH1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA4/TO/FLAG-hPMR1-Tev-Bio

    Plasmid

    #80125

    Purpose

    Expresses human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tags

    Depositor

    Daniel Schoenberg

    Article

    Gu et al RNA. 2012 Jun;18(6):1186-96. doi: 10.126

    Insert

    PXDNL-003 (PXDNL Human)
    

    Use

    Tags

    FLAG and biotinylation

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pK-SAN1-Flag

    Plasmid

    #117163

    Purpose

    Expresses Flag-tagged SAN1 nuclease in mammalian cells.

    Depositor

    Ian Macara

    Article

    Andrews et al Nat Commun. 2018 Jul 3;9(1):2592. doi: 1

    Insert

    SAN1 (FAM120B Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. CKAP2 pHAT

    Plasmid

    #175946

    Purpose

    Bacterial expression of mmCKAP2 pHAT N-terminal 6xHis-tag, C-terminal Strep-tagII

    Depositor

    Susanne Bechstedt

    Article

    McAlear et al Elife. 2022 Jan 14;11. pii: 72202. doi:

    Insert

    CKAP2 (Ckap2 Mouse)
    

    Use

    Tags

    6-His and StrepTagII

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-ABKAR-Kras

    Plasmid

    #84637

    Purpose

    Unimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    Cerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
    

    Use

    Tags

    6xHis, C-terminal targeting sequence from Kras, C…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. WFS1 mRFP-N2 (891)

    Plasmid

    #62018

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    WFS1 (WFS1 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pNIA-CEN-Venus-FLAG-LINXa4-Bre1

    Plasmid

    #73618

    Purpose

    Visualizing the nuclear translocation of LINXa4-Bre1 in yeast.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    Venus
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A

    Promoter

    ADH1

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-PKCsub(T/A)-YPet-NES

    Plasmid

    #138208

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKCsub(T/A)-YPet-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    Sept. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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