Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 12,176 results for: LEA

Clear keywords
Showing: 2081 - 2100 of 12176 results

  3. cycLuc_TEVS

    Plasmid
    #119207
    Purpose
    Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Publication
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cyclic luciferase with TEVs cleavage site
    Use
    Luciferase
    Tags
    6xHis and AU1 tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    119207-plasmid-map-sequence-id-232048

    Enlarge

  4. CBX7

    Plasmid
    #25241
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX7_04 (CBX7 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    4 Articles

  5. pαH-S-GSAS-2P-OMICRON

    Plasmid
    #180593
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180593-plasmid-map-sequence-id-352871

    Enlarge

  6. pEGFP C3-YAP-deltaC

    Plasmid
    #21126
    Depositor
    Marius Sudol
    Publication
    Tsutomu Oka & Marius et al
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Yes-kinase associated protein (YAP1 Human)
    Tags
    EGFP-C3
    Expression
    Bacterial and Mammalian
    Mutation
    5 Carboxy-terminal amino acids -FLTWL are deleted
    Available since
    June 23, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  12. pcDNA3-endo-EKAR4

    Plasmid
    #205513
    Purpose
    Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.
    Depositor
    Jin Zhang
    Publication
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    endo-EKAR4
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    205513-plasmid-map-sequence-id-409396

    Enlarge

  14. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid
    #104909
    Purpose
    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    104909-plasmid-map-sequence-id-206071

    Enlarge

  18. pDN80

    Plasmid
    #61349
    Purpose
    LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.
    Depositor
    Barbara Di Ventura, Roland Eils
    Publication
    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.
    Type
    Empty backbone
    Tags
    AsLOV2 wt and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    61349-plasmid-map-sequence-id-99714

    Enlarge

Showing: 2081 - 2100 of 12176 results