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160,787 results

Showing: 2001 - 2020 of 160787 results

  7. TFORF3460

    Plasmid
    #144936
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PSMB1 (PSMB1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144936-plasmid-map-sequence-id-404495

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  9. pLCHKO_HPRT1_exon3_1_Lb

    Plasmid
    #155053
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 3
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155053-plasmid-map-sequence-id-308427

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  10. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide3

    Plasmid
    #125430
    Purpose
    CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    125430-plasmid-map-sequence-id-251264

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  12. Zebrafish aBb crystallin 2kb promoter/AcGFP

    Plasmid
    #98099
    Purpose
    Zebrafish aBb crystallin 2kb promoter segment driving GFP expression
    Depositor
    Mason Posner
    Article
    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717
    Insert
    Alpha Bb-crystallin promoter 2 kb fragment, Danio rerio (cryabb Zebrafish)
    Use
    Gfp expressing
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Danio alpha Bb-crystallin 2 kb fragment (-2092/-1)
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    98099-plasmid-map-sequence-id-193847

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  16. pCMV-PE6b

    Plasmid
    #207852
    Purpose
    Mammalian expression of PE6b prime editor
    Depositor
    David Liu
    Article
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Insert
    PE6b
    Use
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207852-plasmid-map-sequence-id-408170

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  18. AIMTOR T757

    Plasmid
    #140828
    Purpose
    AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells
    Depositor
    Anne Bonnieu
    Article
    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1
    Insert
    AIMTOR T757 (ULK1 Human)
    Use
    Tags
    NES Nuclear export signal
    Expression
    Mammalian
    Mutation
    The insert comprises only a small sequence of hum…
    Promoter
    Available since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    140828-plasmid-map-sequence-id-305141

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  19. pGZ108 (pFA6a-3FLAG-MNase-kanMX6)

    Plasmid
    #70231
    Purpose
    ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope
    Depositor
    Steven Henikoff
    Article
    Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.
    Insert
    3xFLAG tag and aa 83-231 of Mnase
    Use
    Yeast genomic targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    70231-plasmid-map-sequence-id-129162

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Showing: 2001 - 2020 of 160787 results