160,864 results

1. pPB-cT3G-cERP2-EZH2

   Plasmid

   #222528

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of EZH2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgLATS1/2-2

   Plasmid

   #229432

   Purpose

   knockout of LATS1 and LATS2

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgLATS1/2-2 (LATS1, LATS2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6;bU6;EFS

   Available since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP1

   Plasmid

   #184556

   Purpose

   Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

   Depositor

   Sandeep Prabhu

   Article

   Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

   Insert

   Kirsten rat sarcoma virus (Kras Mouse)
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-MBP-TEVpCS-ADGRA3 CTFdeltaTA-FLAG

   Plasmid

   #217713

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRA3 (ADGRA3 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-743

   Promoter

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcdna 3.1 DSG-3 Tailless Control

   Plasmid

   #182718

   Purpose

   expresses a truncated force-insensitive DSG-3 TSmod

   Depositor

   Daniel Conway

   Article

   Jin et al bioRxiv [Preprint]. 2024 May 6:2024.05.0

   Insert

   desmoglein-3 (Dsg3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   TSmod inserted at G726, downstream C-terminal tai…

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5 TEX264wt-cGFP

   Plasmid

   #220211

   Purpose

   Mammalian expression of wild type TEX264 fused at C-terminus to GFP

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Tags

   Strep-strep-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-MBP-TEVpCS-ADGRA2 CTFdeltaTA-FLAG

   Plasmid

   #217712

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRA2 (ADGRA2 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-752

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-MBP-TEVpCS-ADGRA1 CTFdeltaTA-FLAG

   Plasmid

   #217711

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRA1 (ADGRA1 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-6

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving

   Plasmid

   #204811

   Purpose

   Bacterial expresison of Enterovirus A71 2A protease

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   A71 2A protease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pHYX137

    Plasmid

    #202814

    Purpose

    Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.

    Depositor

    Jean-Félix Dallery

    Article

    Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScPCK1

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHYX138

    Plasmid

    #202815

    Purpose

    Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter ADH2. URA3 selection.

    Depositor

    Jean-Félix Dallery

    Article

    Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScADH2

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. BPK2014-Cas12i-Max

    Plasmid

    #188276

    Purpose

    prokaryotic expression

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    Cas12i-Max
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF1737

    Plasmid

    #142839

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZBTB7B (ZBTB7B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST_FLAG-HUWE1_delta169-189

    Plasmid

    #187136

    Purpose

    mammalian cell expression of FLAG-HUWE1 delta169-189

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    delta169-189

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUW-TetO-CREM

    Plasmid

    #178442

    Purpose

    doxycycline-inducible expression of Human CREM in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    CREM (CREM Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. SspB-iRFP-DHPH1-TRIO

    Plasmid

    #176119

    Purpose

    Heterodimerization with iLID, Rac1 and RhoG activation

    Depositor

    Dorus Gadella

    Insert

    TRIO (TRIO Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUASTLOTattB_mCherry::T48-Baz::vhhGFP4

    Plasmid

    #163928

    Purpose

    Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. VanR-wt

    Plasmid

    #153339

    Purpose

    Wild type VanR from Caulobacter crescentus. The gene is under the control of the TEF1 promoter

    Depositor

    Michael Jensen

    Article

    et al Biotechnology Notes, Volume 1, 2020, Pag

    Insert

    VanR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBW002

    Plasmid

    #131591

    Purpose

    Vector with aTc inducible M. laminosus Fd1 without BbsI restriction sites in the vector backbone

    Depositor

    Jonathan Silberg

    Article

    Wu et al bioRxiv May 23, 2019

    Insert

    Mastigocladus laminosus ferredoxin
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBW004

    Plasmid

    #131805

    Purpose

    Vector constitutively expressing Zea mays FNR and Z. mays SIR with an insertion following BCD13 and preceeding the zmFNR gene deleted 'GGAGAGCATTAGGTCTCG'

    Depositor

    Jonathan Silberg

    Article

    Wu et al bioRxiv May 23, 2019

    Insert

    Zea mays FNR and Z. mays SIR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge