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Addgene

pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6
(Plasmid #177849)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177849 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pETDuet1
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    photoreceptor cell-specific nuclear receptor
  • Alt name
    NR2E3 (nuclear receptor subfamily 2, group E, member 3)
  • Species
    H. sapiens (human)
  • Entrez Gene
    NR2E3 (a.k.a. ESCS, PNR, RNR, RP37, rd7)
  • Tags / Fusion Proteins
    • MBP (N terminal on insert)
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pET Upstream
  • 3′ sequencing primer DuetDOWN1
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6 was a gift from Karsten Melcher (Addgene plasmid # 177849 ; http://n2t.net/addgene:177849 ; RRID:Addgene_177849)
  • For your References section:

    The crystal structure of the orphan nuclear receptor NR2E3/PNR ligand binding domain reveals a dimeric auto-repressed conformation. Tan MH, Zhou XE, Soon FF, Li X, Li J, Yong EL, Melcher K, Xu HE. PLoS One. 2013 Sep 12;8(9):e74359. doi: 10.1371/journal.pone.0074359. eCollection 2013. 10.1371/journal.pone.0074359 PubMed 24069298