pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6
(Plasmid
#177849)
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PurposeBacterial Expression of PNR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 177849 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepETDuet1
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namephotoreceptor cell-specific nuclear receptor
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Alt nameNR2E3 (nuclear receptor subfamily 2, group E, member 3)
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SpeciesH. sapiens (human)
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Entrez GeneNR2E3 (a.k.a. ESCS, PNR, RNR, RP37, rd7)
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Tags
/ Fusion Proteins
- MBP (N terminal on insert)
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pET Upstream
- 3′ sequencing primer DuetDOWN1 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6 was a gift from Karsten Melcher (Addgene plasmid # 177849 ; http://n2t.net/addgene:177849 ; RRID:Addgene_177849) -
For your References section:
The crystal structure of the orphan nuclear receptor NR2E3/PNR ligand binding domain reveals a dimeric auto-repressed conformation. Tan MH, Zhou XE, Soon FF, Li X, Li J, Yong EL, Melcher K, Xu HE. PLoS One. 2013 Sep 12;8(9):e74359. doi: 10.1371/journal.pone.0074359. eCollection 2013. 10.1371/journal.pone.0074359 PubMed 24069298