Addgene

We narrowed to 4,957 results for: hwa

Showing: 1981 - 2000 of 4957 results

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pLKO.1 puro shRNA E-cadherin

Plasmid

#18801

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

E-cadherin shRNA (CDH1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 22, 2008

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-PD-L2

Plasmid

#223587

Purpose

Lentiviral expression of human PD-L2 with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

PD-L2 (PDCD1LG2 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-vioABECD

Plasmid

#66535

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoter

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Consensus T7

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pWZL Blast DN E-cadherin

Plasmid

#18800

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

Dom neg E-cadherin (Cdh1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

truncated form of E-cadherin (dominant negative) …

Promoter

Available since

July 15, 2008

Availability

Academic Institutions and Nonprofits only

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DsRed-Rab5 WT

Plasmid

#13050

Depositor

Richard Pagano

Article

Sharma et al J Biol Chem. 2003 Feb 28. 278(9):7564-72

Insert

RAB5A (Canis familiaris) (RAB5A Canis familiaris)

Use

Tags

DsRed2 and HA

Expression

Mammalian

Mutation

asparagine 3 changed to serine (neutral); serine …

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-NGLY1-Puro

Plasmid

#181916

Purpose

Lentiviral plasmid that directs the expression of NGLY1 in mammalian cells.

Depositor

Scott Dixon

Article

Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

N-glycanase 1 (NGLY1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pTwist-SFFV-NFE2L1-Puro

Plasmid

#181917

Purpose

Lentiviral plasmid that directs the expression of NFE2L1/Nrf1 in mammalian cells.

Depositor

Scott Dixon

Article

Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

NFE2L1 (NFE2L1 Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-yomRuby3-SpHIS5

Plasmid

#204287

Purpose

For C-terminal tagging using yeast optimized mRuby3

Depositor

Linda Huang

Article

Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

Insert

pFA6-yomRuby3-SpHIS5

Use

Tags

yomRuby3

Expression

Yeast

Mutation

Promoter

none

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen_OmpA-mCherry

Plasmid

#182390

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen and ompA-mCherry fusions

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-mNeongreen-His
OmpA-mCherry

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Opto-beta2AR

Plasmid

#106063

Purpose

Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-beta2AR; Opto-beta2AR; F5; 716)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-beta2AR

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Bmi1-overexpression

Plasmid

#21577

Depositor

Sally Temple

Article

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Insert

Bmi-1 (Bmi1 Mouse)

Use

Lentiviral

Tags

ires-eGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only

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FLAG SMG1 D2335A/N2340K

Plasmid

#199575

Purpose

Expresses FLAG-tagged kinase dead SMG1

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

D2335A and N2340K

Promoter

CMV

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mArf1(Q71L)-HA

Plasmid

#79410

Purpose

Expresses C-terminally HA-tagged Arf1(Q71L) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf1 (Arf1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Glutamine 71 to Leucine

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGrRenilla

Plasmid

#118060

Purpose

GBPart - GreenRenilla Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Green Renilla

Use

Luciferase and Synthetic Biology; Gbpart

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCRY2FL(deltaNLS)-mCherryN1

Plasmid

#26871

Purpose

Mammalian expression of cytoplasmic CRY2-mCherry

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

NLS sequences deleted

Promoter

CMV

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pbGH

Plasmid

#118061

Purpose

GBPart - Bovine growth hormone terminator

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

bgH_poly(A)

Use

Synthetic Biology; Gbpart

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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mEmerald-MyosinIIA-N-14

Plasmid

#54191

Purpose

Localization: Cytoskeleton, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

Insert

MyosinIIA

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
myc-mTOR (1750-2549 aa)

Plasmid

#21747

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1750-2549) (Mtor Rat)

Use

Tags

myc

Expression

Mammalian

Mutation

amino acids 1750-2549

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-Tag4A-MITF-M (wt)

Plasmid

#31151

Depositor

Yardena Samuels

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

MITF-M (MITF Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

WT

Promoter

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only

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E[beta]P

Plasmid

#24313

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

EF1alpha-betacatenin(deltaGSK) // SV40-PuroR (Ctnnb1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Sites phosphorylated by GSK-3beta have been mutat…

Promoter

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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