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pX459-dTLN1 exon

Plasmid

#176033

Purpose

A vector for the CRISPR-Cas9 system targeting an exon of dog TLN1 (talin1)

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

A gRNA targeting the dog TLN1 gene and the cDNA of Cas9 (TLN1 )

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

Plasmid

#172982

Purpose

control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

control sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLV-hIP-dCas9-BSD

Plasmid

#183231

Purpose

Lentiviral vectors with the human insulin promoter driving expression of dCas9, in addition to a U6-driven sgRNA

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Human insulin promoter

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-Cas9GFP-W

Plasmid

#200100

Purpose

Lentiviral vector expressing Cas9 fused with GFP at the C-terminus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

GFP

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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All-in-one lenti-ABE8e-NG

Plasmid

#200447

Purpose

Lentivirus vector for base editing

Depositor

Benjamin Cravatt

Article

Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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All-in-one lenti-BE4-NG

Plasmid

#200448

Purpose

Lentivirus vector for base editing

Depositor

Benjamin Cravatt

Article

Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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LentiCROP-PuroR-P2A-GFP-U6

Plasmid

#200029

Purpose

Lentiviral CROP-seq vector backbone with PuroR-P2A-GFP for cloning DNA Ticker Tapes and U6 for pegRNAs

Depositor

Jay Shendure

Type

Empty backbone

Use

Lentiviral

Tags

PuroR-P2A-GFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PX459-gR2A_Hinge

Plasmid

#124811

Purpose

Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.

Depositor

Ferenc Scheeren, Martijn Verdoes

Article

van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

Insert

Cas9 – gRNA_R2A_hinge

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Bacterial and Mammalian

Mutation

R166H in PuroR (please see depositors comment bel…

Promoter

pUC

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast

Plasmid

#71217

Purpose

dCas9 fused to human DNMT3B catalytic domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3B catalytic domain

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pDY0312 PhiBT Integrase

Plasmid

#179111

Purpose

PhiBT Integrase expression vector

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

PhiBT Integrase

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCas-pML-1B_6xHis-Cas12m

Plasmid

#192282

Purpose

MmCas12m fused to His-tag for protein purification

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmCas12m

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

Plasmid

#197024

Purpose

Vector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoter

Depositor

Xiangfeng Kong

Article

Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

Insert

humanized OsCas12f1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CBh, CMV, hU6

Availability

Academic Institutions and Nonprofits only

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pCBh_3x Flag-NLS-RhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

Plasmid

#197025

Purpose

Vector encoding a human codon-optimized RhCas12f1 driven by CBh promoter, guide RNAs compatible with RhCas12f1 driven by hU6, and mCherry driven by CMV promoter

Depositor

Xiangfeng Kong

Article

Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

Insert

humanized RhCas12f1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CBh, CMV, hU6

Availability

Academic Institutions and Nonprofits only

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pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

Plasmid

#197026

Purpose

Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter

Depositor

Xiangfeng Kong

Article

Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

Insert

humanized enOsCas12f1

Use

Tags

Expression

Mammalian

Mutation

D52R / T132R

Promoter

CBh, CMV, hU6

Availability

Academic Institutions and Nonprofits only

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pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

Plasmid

#197027

Purpose

Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter

Depositor

Xiangfeng Kong

Article

Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

Insert

humanized enRhCas12f1

Use

Tags

Expression

Mammalian

Mutation

L270R

Promoter

CBh, CMV, hU6

Availability

Academic Institutions and Nonprofits only

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LV-PE_CO-Mini

Plasmid

#190338

Purpose

Lentiviral vector expressing PE_CO-Mini (Prime editor with codon-optimized and truncated Reverse transcriptase)

Depositor

Rasmus Bak

Article

Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

Insert

PE_CO-Mini, ngRNA and pegRNA for HEK3 CTTins

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

Plasmid

#115210

Purpose

Expresses AspCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

AspCas13

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pC0061 PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI

Plasmid

#115211

Purpose

Expresses PsmCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PsmCas13

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI

Plasmid

#115267

Purpose

Expresses LbuCas13a from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

LbuCas13a

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-pU6-sgRNA

Plasmid

#159174

Purpose

Vector B encodes pAAV-pMecp2-dSaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of dSaCas9-sgRNA in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pMecp2

Availability

Academic Institutions and Nonprofits only

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