pAAV-pMecp2-dSaCas9-pU6-sgRNA
(Plasmid
#159174)
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PurposeVector B encodes pAAV-pMecp2-dSaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of dSaCas9-sgRNA in neurons
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159174 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepX601 (Addgene #61591)
- Total vector size (bp) 6889
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Modifications to backbonereplaced CMV promoter with pMecp2; replace bGHpA with spA; replaced SaCas9 with dSaCas9
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Vector typeMammalian Expression, AAV, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedSaCas9
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SpeciesSynthetic
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Insert Size (bp)3260
- Promoter pMecp2
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site BamH (unknown if destroyed)
- 5′ sequencing primer GCAGGTTGTAGTCGAACAGCAGCT
- 3′ sequencing primer CAGCACAGACATTCTGGGCAACCT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-pMecp2-dSaCas9-pU6-sgRNA was a gift from Chung Tin (Addgene plasmid # 159174 ; http://n2t.net/addgene:159174 ; RRID:Addgene_159174) -
For your References section:
Targeted Transgene Activation in the Brain Tissue by Systemic Delivery of Engineered AAV1 Expressing CRISPRa. Lau CH, Ho JW, Lo PK, Tin C. Mol Ther Nucleic Acids. 2019 Jun 7;16:637-649. doi: 10.1016/j.omtn.2019.04.015. Epub 2019 Apr 23. 10.1016/j.omtn.2019.04.015 PubMed 31108320