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Plasmid#211851PurposeCDS for HR1b degron sequence in domestication vectorDepositorInsertHR1b
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-LRR-NEL (B4-B5)
Plasmid#211859PurposeCDS for E3-DART ligase in domestication vectorDepositorInsertLRR-NEL
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-GFP control (B3-B5)
Plasmid#211848PurposeGFP CDS in domestication vectorDepositorInsertGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-N-L-mCherry2 (B5)
Plasmid#211849PurposeCDS for mCherry in domestication vectorDepositorInsertmCherry
UseSynthetic BiologyTagsmCherryExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-N-L-HR1b (B4-B5)
Plasmid#211852PurposeCDS for HR1b degron sequence in domestication vectorDepositorInsertHR1b
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK1051
Plasmid#209541PurposeBTK Type 5 homology arm for Golden Gate assemblyDepositorInsertRecombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK1059
Plasmid#209549PurposeBTK Type 5 homology arm for Golden Gate assemblyDepositorInsertDownstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCOBL-GW
Plasmid#209376PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, driven by COBL promoterDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLBD16-GW
Plasmid#209377PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, driven by LBD16 promoterDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAB1-GW
Plasmid#209371PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, driven by CAB1 promoterDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest_U6_A2_CG2_erbb2_gRNA171
Plasmid#199385PurposepDEL133; Gateway Destination vector for cell-type specific CRISPR encoding erbb2 sgRNADepositorInserterbb2 sgRNA 171
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP_sgRNA
Plasmid#190128PurposeLevel 0 MoClo plasmid for Golden Gate cloning of sgRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP_dCas9
Plasmid#190131PurposeLevel 0 MoClo plasmid containing dCas9DepositorInsertdCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pND005
Plasmid#200951PurposeYeast two-hybrid destination vector for Golden Gate cloningDepositorTypeEmpty backboneUseYeast two-hybridTagsExpressionMutationPromoterGAL4Available sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJBL3907
Plasmid#193140PurposeClostridium beijerinckii pfl ZTP riboswitch with sfGFP reporterDepositorInsertClostridium beijerinckii (Cbe) pfl ZTP riboswitch with sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pITC
Plasmid#192856PurposeCloning and expression of transcription factor gene under control of naringenin inducible promoterDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPfdeARAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_E2Aq(2nd)
Plasmid#190911Purpose2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assemblyDepositorInsertE2A
UseTagsExpressionMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_O2Aq(2nd)
Plasmid#190918Purpose2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assemblyDepositorInsertO2A
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_P2Aq(1st)
Plasmid#190912Purpose2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assemblyDepositorInsertP2A
UseTagsExpressionMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_O2Aq(1st)
Plasmid#190917Purpose2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assemblyDepositorInsertO2A
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only