pLP-TRE2-hSox2
(Plasmid
#49392)
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Purposeinducible (dox) expression of hSox2
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49392 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLP-TRE2
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3900
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Vector typeMammalian Expression, Cre/Lox ; donor vector
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSox2
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Alt namehuman Sry
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SpeciesH. sapiens (human)
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Insert Size (bp)953
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MutationTGA (stop) to CCA (Pro) at nt 1027 with respect to GenBank ID BC013923
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GenBank IDBC013923
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Entrez GeneSOX2 (a.k.a. ANOP3, MCOPS3)
- Promoter Tet-responsive
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Tag
/ Fusion Protein
- 6xHN (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer LNCX_primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
hSox2 was transferred from the donor vector (pDNR-hSox2, Addgene plasmid #49389) to the acceptor vector using Cre-mediated recombination at the LoxP sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLP-TRE2-hSox2 was a gift from Randall McKinnon (Addgene plasmid # 49392 ; http://n2t.net/addgene:49392 ; RRID:Addgene_49392)
Map uploaded by the depositor.