pTH-NG(T)-mCherry
(Plasmid
#49627)
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Purpose(Empty Backbone) Destination Vector for TALEColor-mCherry
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49627 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA4/TO
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Backbone manufacturerInvitrogen
- Backbone size (bp) 5500
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Vector typeMammalian Expression, TALEN
- Promoter CMV
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Selectable markersHygromycin
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Tag
/ Fusion Protein
- mCherry (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This destination vector for TALEs is compatible with the Zhang Lab TALE Toolbox.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTH-NG(T)-mCherry was a gift from Thoru Pederson (Addgene plasmid # 49627 ; http://n2t.net/addgene:49627 ; RRID:Addgene_49627) -
For your References section:
Visualization of repetitive DNA sequences in human chromosomes with transcription activator-like effectors. Ma H, Reyes-Gutierrez P, Pederson T. Proc Natl Acad Sci U S A. 2013 Dec 9. 10.1073/pnas.1319097110 PubMed 24324157