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Plasmid#64238PurposeLentiviral, doxycycline-inducible system to express transgene of interest and EGFP.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterpTetAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only
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mEGFP-C1
Plasmid#54759PurposeLocalization: C1 Cloning Vector, Excitation: 488, Emission: 507DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
Flag-FOXC1
Plasmid#153114PurposeExpression of Flag tagged human FOXC1 in mammalian cellsDepositorInserthuman FOXC1 (FOXC1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-Caspase 1
Plasmid#41552DepositorInsertCaspase-1 (CASP1 Human)
UseTagsMycExpressionMammalianMutationPromoterCMV immediate-early enhancer/promoterAvailable sinceDec. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
dTomato-2xrGBD
Plasmid#129625PurposeVisualization of active RhoADepositorInsertdTomato-2xrGBD
UseTagsExpressionMammalianMutationPromoterCMVdelAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEFIRES-P-ACSL3-mCherry
Plasmid#87158PurposeFluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)DepositorInsertACSL3 (ACSL3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterEF-1-alphaAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
mito-meGFP
Plasmid#172481PurposemeGFP targeted to the mitochondrial matrix with a human COX8 presequenceDepositorInsertMito
UseTagsmeGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-TOP3B
Plasmid#225470PurposeExpress mEGFP-fusion protein of TOP3BDepositorInsertTOP3B (TOP3B Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-HA-UBE3A-IRES-Puro
Plasmid#107349PurposeExpresses HA tagged UBE3A in mammalian cells.DepositorInsertUBE3A (UBE3A Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBABE-hygro-hTERT
Plasmid#1773PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.DepositorInserthTERT (TERT Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-mScarlet-I
Plasmid#98827PurposeIn vivo visualization of lysosomes (can be used for colocalization studies)DepositorArticleInsertLamp1
UseTagsmScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-BRCA1
Plasmid#115491PurposeEGFP-BRCA1 expression vector. Parton clone BXYDepositorInsertBRCA1 (BRCA1 Human)
UseTagsEGFPExpressionMammalianMutationS1009R (See depositor comments)PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-PSD95.FingR-eGFP-CCR5TC
Plasmid#125693PurposeGlobal labeling of excitatory synapses (green fluorescence)DepositorInsertPSD95.FingR-eGFP-CCR5TC
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)
Plasmid#139998PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…PromoterCAGAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-tetO-NB
Plasmid#197092PurposePiggyBac plasmid with tet-inducible expression of transcription factors for sensory neuron differentiationDepositorUseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDG461
Plasmid#100902PurposeSpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.DepositorInsertshumanized CRISPR associated protein 9 Nickase
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAG and T2A-EGFPExpressionMammalianMutationD10A mutant converts to NickasePromoterCBh and U6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCh-Climp63
Plasmid#136293Purposeexpression of Climp63DepositorInsertCytoskeletal-linking protein 63 (Ckap4 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-DDX54-FLAG/HA
Plasmid#97062PurposeExpresses DDX54-FLAG/HA (C-terminal tagged) in mammalian cellsDepositorInsertDDX54 (DDX54 Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE
Plasmid#125712PurposeExpresses an optimized mosquito OPN3 rhodopsin in-frame with mScarletDepositorHas ServiceAAV1 and AAV5InserteOPN3
UseAAVTagsRho1D4 and mScarletExpressionMammalianMutationdeleted amino acids 331-429Promotershort version of αCamKinase II promoter (0.4kb)Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
p27-IRES-mCherry
Plasmid#80143Purposeretroviral expression of p27 and mCherryDepositorInsertp27 (Cdkn1b Mouse)
UseRetroviralTagsIRES-mCherryExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only