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PurposeAAV expression of ChrimsonR-GFP under the Syn promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58806 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV
- Backbone size w/o insert (bp) 4698
- Total vector size (bp) 6483
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsStbl3 at 30C (use carbenicillin if using Stbl3) OR DH5a at 37C
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameChrimsonR-GFP
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Alt nameChrimson K176R mutant
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Alt nameChR88m19-GFP
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SpeciesChlamydomonas noctigama
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Insert Size (bp)1785
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MutationK176R
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GenBank IDKF992060
- Promoter Syn
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CTGCGTATGAGTGCAAG
- 3′ sequencing primer cagcgtatccacatagcg (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid is sequenced completely by the depositing lab except for part of the origin and 5' ITR. Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-Syn-ChrimsonR-GFP was a gift from Edward Boyden (Addgene plasmid # 58806 ; http://n2t.net/addgene:58806 ; RRID:Addgene_58806) -
For your References section:
Independent optical excitation of distinct neural populations. Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, Boyden ES. Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 2014 Feb 9. 10.1038/nmeth.2836 PubMed 24509633