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Plasmid#161896PurposeMultisite Gateway (attR4-attR3) vector for mammalian protein expressionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only
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pAAV-G12-mTGFB1
Plasmid#219553PurposemTGFB1 expression in mammlian cells. pAAV vector. Reporter plasmid.DepositorInsertmTGFB1 (Tgfb1 Mouse)
UseAAVTagsExpressionMammalianMutationPromoter12xUAS with TATA-box minimal promoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT2-shP53
Plasmid#124261PurposeExpresses shRNA targeting P53. Construct has inverted repeats to be used in Sleeping beauty system.DepositorInsertshP53
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
AbVec2.0-mIghg2b
Plasmid#127155PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2b isotypeDepositorInsertIghg2b (Ighg2b Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE TRE dCas9-KRAB
Plasmid#50917PurposeTet-regulatable dCas9-KRAB 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and KRABExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterTREAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1_Puro
Plasmid#125594PurposeLentiviral expression of sgRNA with GFP and puromycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for sgRNA expression and EFS promoter…Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEZT-GST-SLFN2
Plasmid#174319PurposeExpression of recombinant GST-SLFN2DepositorInsertGST-SLFN2 (Slfn2 Mouse)
UseBacmam, baculovirusTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
cfSGFP2-col4a1
Plasmid#160317PurposecfSGFP2-tagged collagen4a1 for mammalian expressionDepositorInsertCOL4A1 (COL4A1 Human)
UseTagscfSGFP2ExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFAP-Cre
Plasmid#24704DepositorInsertCre
UseTagsExpressionMammalianMutationPromoterGFAP (glial fibrillary acidic protein)Available sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNL(CMV)Rep78-III/CAG/WPREΔU3
Plasmid#190727PurposeExpression of AAV2 Rep78-III in mammalian cellsDepositorInsertAAV2 Rep78-III
UseLentiviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP×2-N1
Plasmid#86775PurposeA mammalian expression vector with double EGFP at C-terminus.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF6 VPS52-V5-6His
Plasmid#175096PurposeV5 tagged VPS52 is expressed in mammalian cellsDepositorInsertVPS52 (VPS52 Human)
UseTagsV5ExpressionMammalianMutationN666D- please see depositor commentPromoterAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIR-CMV-SCN2A-Variant-1-IRES-mScarlet
Plasmid#162279PurposeEukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN2A Variant 1, stabilized (SCN2A Human)
UseTagsIRES-mScarletExpressionMammalianMutationIVS intron inserted after bp4551.PromoterCMVAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam p53 wt
Plasmid#16434DepositorInsertp53 (TP53 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-DVL1-WT
Plasmid#194586PurposeMammalian Expression of mEGFP-DVL1 WT Fusion ProteinDepositorInsertDVL1 (DVL1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV CEPIA3mt
Plasmid#58219PurposeGreen fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)DepositorInsertCEPIA3mt
UseTagsmycExpressionMammalianMutationGCaMP2 N263Y E282V E334D M339LPromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-NGN2-EMX1 puro-BFP
Plasmid#182312PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, puromycin selection, BFPUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIP-AMPKa1_WT
Plasmid#79010PurposeFor mammalian expression of WT AMPK alpha1.DepositorInsertAMPK alpha 1 (PRKAA1 Human)
UseTagsExpressionMammalianMutationPromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-sadCas9-VP64
Plasmid#115790PurposeAn AAV vector expressing S. aureus dCas9 fused to VP64.DepositorInsertSa-dCas9
UseAAVTagsNLS and NLS-VP64ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Neo (657-2)
Plasmid#17447Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, NeoDepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only