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PurposecfSGFP2-tagged collagen4a1 for mammalian expression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 160317 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneEGFP-N1
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Backbone manufacturerClonetech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCOL4A1
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SpeciesH. sapiens (human)
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Entrez GeneCOL4A1 (a.k.a. BSVD, BSVD1, COL4A1s, PADMAL, RATOR)
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Tag
/ Fusion Protein
- cfSGFP2
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains a T824P mutation in the insert (lies within the Mature Domain). This variant is not known to affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
cfSGFP2-col4a1 was a gift from Ikuo Wada (Addgene plasmid # 160317 ; http://n2t.net/addgene:160317 ; RRID:Addgene_160317) -
For your References section:
Visualization of Procollagen IV Reveals ER-to-Golgi Transport by ERGIC-independent Carriers. Matsui Y, Hirata Y, Wada I, Hosokawa N. Cell Struct Funct. 2020 Jul 23;45(2):107-119. doi: 10.1247/csf.20025. Epub 2020 Jun 18. 10.1247/csf.20025 PubMed 32554938
Map uploaded by the depositor.
