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ERK-KTR-mStrawberry reporter

Plasmid

#158686

Purpose

This plasmid is a ERK pathway reporter. It has CMV promoter driving the expression of ERK-KTR fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMK286 (mAID-NeoR)

Plasmid

#72824

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Neo

Use

CRISPR

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-SOX9

Plasmid

#141404

Purpose

Inducible Expression of SOX9

Depositor

Lorenz Studer

Article

Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

Insert

SOX9 (SOX9 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-Cre-blast

Plasmid

#179390

Purpose

Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

Insert

mCherry (P2A) Cre recombinase

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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PKA-KTR-mStrawberry reporter

Plasmid

#158688

Purpose

This plasmid is a PKA pathway reporter. It has CMV promoter driving the expression of PKA-KTR fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-EEA1 wt

Plasmid

#42307

Depositor

Silvia Corvera

Article

Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

Insert

EEA1 (EEA1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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mCherry2-C1

Plasmid

#54563

Purpose

Localization: C1 Cloning Vector, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

mCherry2

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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HA-MCPIP1

Plasmid

#127459

Purpose

Expressing human MCPIP1 in mammalian cells

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Cre:GFP

Plasmid

#13776

Purpose

Mammalian expression of Cre recombinase fused to GFP

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Cre-GFP fusion protein

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pSAC5.02

Plasmid

#16077

Depositor

Lonny Levin, Jochen Buck

Article

Buck et al Proc Natl Acad Sci U S A. 1999 Jan 5. 96

Insert

soluble adenylyl cyclase (Adcy10 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
FUdeltaGW-rtTA

Plasmid

#19780

Purpose

3rd generation lentiviral vector

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

reverse tetracycline transactivator

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2009

Availability

Academic Institutions and Nonprofits only

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NFAT luciferase reporter

Plasmid

#10959

Depositor

Toren Finkel

Article

Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

Insert

IL-2 promoter (IL2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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Lentiviral-TOP-dGFP-reporter

Plasmid

#14715

Purpose

Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

LEF-1/TCF-responsive promoter (Ctnnb1 )

Use

Lentiviral

Tags

d2 eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH N90-beta-catenin

Plasmid

#86499

Purpose

Mammalian expression of Myc tagged N90 beta-catenin

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

beta-catenin (CTNNB1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Deletion of amino acids 1-90; constitutively acti…

Promoter

EF1a

Available since

Feb. 16, 2017

Availability

Academic Institutions and Nonprofits only

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mEmerald-Sec61b-C1

Plasmid

#90992

Purpose

Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Article

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Insert

mEmerald-Sec61b (SEC61B Synthetic, Human)

Use

Tags

mEmerald (green fluorescence)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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SF-SON IRES Tomato

Plasmid

#214403

Purpose

Expresses human SOX10, OLIG2, NKX6.2 and tdTomato

Depositor

Tanja Kuhlmann

Article

Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-UbC-rtTA-Ngn2:2A:Ascl1

Plasmid

#127289

Purpose

all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)

Depositor

Fred Gage

Article

Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1

Inserts

Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)

Use

Lentiviral

Tags

Ngn2 and Ascl1 linked via 2A

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTet-IRES-EGFP

Plasmid

#64238

Purpose

Lentiviral, doxycycline-inducible system to express transgene of interest and EGFP.

Depositor

Maria Lung

Article

Shuen et al Cancer Gene Ther. 2015 Feb 27. doi: 10.1

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

pTet

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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mEGFP-C1

Plasmid

#54759

Purpose

Localization: C1 Cloning Vector, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1+-Tornado-split-nLuc

Plasmid

#212612

Purpose

For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-split-nLuc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

C373T mutation in CVB3

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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