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69,384 results

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pcDNA3.1 iCasper T2A HO1

Plasmid

#64278

Purpose

Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Nup88

Plasmid

#64281

Purpose

Mammalian expression of full-length Nup88

Depositor

Birthe Fahrenkrog

Article

Lussi et al Mol Biol Cell. 2011 Apr;22(7):1080-90. d

Insert

Nup88 (NUP88 Human)

Use

Tags

his and myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Nup88(1-550)

Plasmid

#64282

Purpose

Mammalian expression of N-terminal domain of Nup88

Depositor

Birthe Fahrenkrog

Article

Lussi et al Mol Biol Cell. 2011 Apr;22(7):1080-90. d

Insert

Nup88 (NUP88 Human)

Use

Tags

his and myc

Expression

Mammalian

Mutation

N-terminal domain (1-550)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pEGFP-Nup88

Plasmid

#64283

Purpose

GFP-tagged mammalian expression of full-length Nup88

Depositor

Birthe Fahrenkrog

Article

Lussi et al Mol Biol Cell. 2011 Apr;22(7):1080-90. d

Insert

Nup88 (NUP88 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pBa-FRB-3myc-KIF1A tail 391-1698

Plasmid

#64286

Purpose

KIF1A aa 391-1698 with N-terminal FRB and triple myc tags

Depositor

Marvin Bentley, Gary Banker

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

KIF1A (Kif1a, Kif1a Mouse, Rat)

Use

Tags

3myc and FRB

Expression

Mammalian

Mutation

aa 391-1698, 5'end mostly matches rat, 3…

Promoter

chicken Beta Actin

Availability

Academic Institutions and Nonprofits only

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pBa-FRB-3myc-KIF1B beta tail 387-1770

Plasmid

#64287

Purpose

KIF1B beta aa 387-1770 with N-terminal FRB and triple myc tags

Depositor

Marvin Bentley, Gary Banker

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

KIF1B beta (Kif1b Mouse, Rat)

Use

Tags

3myc and FRB

Expression

Mammalian

Mutation

aa 387-1770, small portion of 5'end matches …

Promoter

chicken Beta Actin

Availability

Academic Institutions and Nonprofits only

Enlarge
pBa-FRB-3myc-KIF13A tail 361-1749

Plasmid

#64288

Purpose

KIF13A aa 361-1749 with N-terminal FRB and triple myc tags

Depositor

Marvin Bentley, Gary Banker

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

KIF13A (Kif13a Mouse)

Use

Tags

3myc and FRB

Expression

Mammalian

Mutation

aa361-1749

Promoter

chicken Beta Actin

Availability

Academic Institutions and Nonprofits only

Enlarge
pBa-FRB-3myc-KIF13B tail 442-1826

Plasmid

#64289

Purpose

KIF13A aa 442-1826 with N-terminal FRB and triple myc tags

Depositor

Marvin Bentley, Gary Banker

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

KIF13B (Kif13b Mouse)

Use

Tags

3myc and FRB

Expression

Mammalian

Mutation

aa 442-1826

Promoter

chicken Beta Actin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLe6Δ -hLP-mcs/(EGFP-IresPuro-hInt)

Plasmid

#64314

Purpose

chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells

Depositor

Noriyuki Tsumaki

Article

Outani et al PLoS One. 2013 Oct 16;8(10):e77365. doi:

Inserts

COL11A2 promoter (COL11A2 Human)
COL11A2 enhancer (COL11A2 Human)

Use

Lentiviral

Tags

EGFP and IRES-PURO

Expression

Mammalian

Mutation

N/A

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP(C3)-Nup153-C (880-1475)

Plasmid

#64317

Purpose

GFP-tagged mammalian expression of C-terminal domain of Nup153

Depositor

Birthe Fahrenkrog

Article

Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

Insert

Nup153 (NUP153 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

C-terminal subunit (880-1475)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pEGFP(C3)-Nup153-N

Plasmid

#64318

Purpose

GFP-tagged mammalian expression of N-terminal domain of Nup153

Depositor

Birthe Fahrenkrog

Article

Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

Insert

Nup153 (NUP153 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

N-terminal domain

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pU6-(BbsI)_CBh-Cas9-T2A-BFP

Plasmid

#64323

Purpose

Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-EBFP2

Expression

Mammalian

Mutation

Promoter

CBh and U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-(BbsI)_CBh-Cas9-T2A-mCherry

Plasmid

#64324

Purpose

Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-mCherry

Expression

Mammalian

Mutation

Promoter

CBh and U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST26-USP47

Plasmid

#64326

Purpose

Mammalian expression and purification of USP47.

Depositor

Grigory Dianov

Article

Parsons et al Mol Cell. 2011 Mar 4;41(5):609-15. doi:

Insert

USP47 (USP47 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES Centrin1 mCherry

Plasmid

#64338

Purpose

Bicistronic expression of mCherry-Centrin1 fusion and Hygro resistance gene

Depositor

Matthieu Piel

Insert

Centrin1

Use

Tags

IRES-Hygro and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES GFP Centrin1 Hygro

Plasmid

#64339

Purpose

Bicistronic expression of GFP-Centrin fusion and Hygro resistance gene

Depositor

Matthieu Piel

Insert

Centrin1

Use

Tags

GFP and IRES-Hygro

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pIRES Centrin1 Cherry Puro

Plasmid

#64340

Purpose

Bicistronic expression of Centrin-mCherry fusion and Puro resistance gene

Depositor

Matthieu Piel

Insert

Centrin1

Use

Tags

IRES-Puro and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pIRES Centrin1 GFP Pur

Plasmid

#64341

Purpose

Bicistronic expression of Centrin-GFP fusion and Puro resistance gene

Depositor

Matthieu Piel

Insert

Centrin1 (CETN1 Human)

Use

Tags

GFP and IRES-Puro

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pIRES Hygro3 LifeAct-mChe

Plasmid

#64343

Purpose

Bicistronic expression of LifeAct-mCherry fusion and Hygro resistance gene

Depositor

Matthieu Piel

Insert

LifeAct

Use

Tags

IRES-Hygro and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pIRES LifeAct-GFP neo3

Plasmid

#64356

Purpose

Bicistronic expression of LifeAct-GFP fusion and neo3 resistance gene

Depositor

Matthieu Piel

Insert

LifeAct-GFP

Use

Tags

IRES-Neo

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only