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Plasmid#36264DepositorInsertDynamin 2 (Dnm2 Mouse)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-FB-dCas9-puro
Plasmid#100547PurposeExpresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 proteinDepositorInsertsN-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
puromycin
UseCRISPRTagsFLAG, Biotin-acceptor-site (Avitag)ExpressionMammalianMutationPromoterHuman EF1aAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV4a-Flag-c-Myc
Plasmid#102625PurposeExpresses c-Myc in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVXPuro-mGFP-LIC1
Plasmid#66600PurposeExpresses Dynein-1 LIC1 as a mGFP fusionDepositorInsertLIC1 (DYNC1LI1 Human)
UseLentiviralTagsmGFPExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Ras-LOCKR-S
Plasmid#216496PurposeFRET-based reporter for Ras activity, untargetedDepositorInsertRas-LOCKR-S
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/CALR wt-FLAG
Plasmid#204532PurposeMammalian expression of human CALR wt-FLAGDepositorInsertCALR wt-FLAG (CALR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
ER-mScarletI
Plasmid#137805PurposeVisualization of the Endoplasmic ReticulumDepositorArticleInsertKDEL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVpuro-CMV-N-EGFP
Plasmid#122848PurposeN-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.DepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF2401
Plasmid#142067PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFE2L3 (NFE2L3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-MYOD1-WT
Plasmid#194570PurposeMammalian Expression of mEGFP-MYOD1 WT Fusion ProteinDepositorInsertMYOD1 (MYOD1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-Tspan4
Plasmid#210323PurposeExpresses Tetraspanin-4 (TSPAN4) as migrasome markerDepositorInsertTetraspanin 4 (Tspan4 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCX-SpiCee-NLS
Plasmid#140900Purposemammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signalingDepositorInsertSpiCee-NLS
UseTagsmRFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFR-mEGFP
Plasmid#203774PurposeNumber and brightness analysis of EGFR oligomerizationDepositorInsertEGFR (EGFR Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pphiLOV3-N1
Plasmid#178973PurposeExpresses phiLOV3 in mammalian cellsDepositorInsertphiLOV3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRY2PHR-mCherryN1
Plasmid#26866PurposeExpresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modulesDepositorInsertCRY2PHR (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
DRD2-Tango
Plasmid#66269PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertDRD2 (DRD2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGs-BFP
Plasmid#127348PurposeExpresses BFP constitutively via the CAGGS promoter in mammalian cellsDepositorInsertBlue Fluorescent Protein
UseTagsExpressionMammalianMutationPromoterCAGGS PromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-3'TwStrep
Plasmid#208618PurposeEmpty vector for mammalian expression, insert Twin Strep tag using BamHI and AflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with Twin Strep tagDepositorTypeEmpty backboneUseTagsTwin StrepExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF3295
Plasmid#144771PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSPDEF (SPDEF Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Kif5c-Myc
Plasmid#186616PurposeThird generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform C fused to Myc tagDepositorInsertKinesin Heavy Chain isoform 5c (KIF5C Human)
UseLentiviralTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only