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Plasmid#191853PurposeExpresses biosensor for cGMP in mammalian cellsDepositorInsertRed cGull
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Bxb1-LP-v2-TC
Plasmid#194326PurposeAAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.DepositorInsertloxP-EBFP2-attP-BleoR-lox251
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-SpG
Plasmid#211549PurposePrime editing in mammalian cellsDepositorInsertPEmax-SpG
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO APEX2-GFP
Plasmid#129640PurposeExpress APEX2-GFP fusion in mammalian cellsDepositorInsertAPEX2-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
V5-miniTurbo-NES_pCDNA3
Plasmid#107170Purposeexpresses V5-tagged miniTurbo in the mammalian cytosolDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionMammalianMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP22
Plasmid#22575DepositorInsertUSP22 (USP22 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac dCas9-VPR
Plasmid#191269PurposepiggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFPDepositorInsertsVPR-dCas9-HA-tagBFP
rtTA3-IRES-PURO
UseCRISPRTagsHA and tagBFPExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
APEX2-csChBP
Plasmid#108876PurposeExpression of APEX2 fused to a conditionally stable nanobody against mCherryDepositorInsertAPEX2-csChBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTipi2.1_Anti-Rho IPI-[1D4] Mouse/Rabbit HC
Plasmid#221365PurposeMammalian expression of Anti-Rho IPI-[1D4] Mouse/Rabbit HC; to be used with Anti-Rho IPI-[1D4] light chain (Plasmid 221366) to make the antibody.DepositorInsertAnti-Rho IPI-[1D4] Mouse/Rabbit HC
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTipi2.1_Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa
Plasmid#221366PurposeMammalian expression of Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa; to be used with Anti-Rho IPI-[1D4] heavy chain (Plasmid 221365) to make the antibody.DepositorInsertAnti-Rho [IPI-1D4] Mouse/Rabbit LC kappa
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
1477 pcDNA3 flag HA Akt1
Plasmid#9021DepositorInsertAkt1 (AKT1 Human)
UseTagsHA and flagExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo
Plasmid#108101PurposeLentiviral expression plasmid of cDNA with neomycin resistance gene (empty vector)DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsFLAG tagExpressionMammalianMutationPromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO hChR2(H134R)-EYFP
Plasmid#55639PurposeFlp-Dependent ChR2-EYFPDepositorInserthChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV G-CEPIA1er
Plasmid#58215PurposeGreen fluorescent indicator for calcium imaging in the endoplasmic reticulumDepositorInsertG-CEPIA1er
UseTagsmycExpressionMammalianMutationG-GECO1.1 E299D M304L D328N N345K F360W G369S E37…PromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
ABE8.20-m
Plasmid#136300Purposeexpresses ABE8.20-m in mammalian cellsDepositorInsertABE8.20-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP37
Plasmid#22602DepositorInsertUSP37 (USP37 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFP hsp70
Plasmid#15215DepositorInserthsp70 (HSPA1A Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
FUGW ECFP
Plasmid#128068PurposeLentiviral expression of ECFPDepositorInsertECFP
UseLentiviralTagsExpressionMammalianMutationnoPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-PGK1
Plasmid#38071DepositorInsertPGK1 phosphoglycerate kinase 1 (PGK1 Human)
UseTagsHIS/FLAG/HAExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
lenti MS2-P65-HSF1_Hygro
Plasmid#61426Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2DepositorInsertMS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only