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pMT Draper-delta ITAM-GFP

Plasmid

#126655

Purpose

Inducible expression of Draper-delta ITAM-GFP mutant in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper with ITAM Tyr residues mutated to Ala

Use

Tags

Expression

Insect

Mutation

Cytoplasmic ITAM Tyr residues mutated to Phe

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pMT Shark-TagBFP

Plasmid

#126658

Purpose

Inducible expression of Shark-TagBFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Shark

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT Crk-mCherry

Plasmid

#126659

Purpose

Inducible expression of Crk-mCherry in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Crk

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT vCrk-GFP

Plasmid

#126661

Purpose

Inducible expression of vCrk truncation in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Crk

Use

Tags

Expression

Insect

Mutation

residues 1-182

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT Ptp69D-GFP

Plasmid

#126663

Purpose

Inducible expression of Ptp69D-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Ptp69D

Use

Tags

Expression

Insect

Mutation

A53S, K105N, and A98T- please see depositor comme…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT FRB-Draper INT-GFP

Plasmid

#126665

Purpose

Inducible expression of a synthetic FRB-Draper receptor in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

FRB-Draper INT domain

Use

Tags

Expression

Insect

Mutation

K986N- see depositor comments below

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT FRB-Draper INT-mCherry

Plasmid

#126666

Purpose

Inducible expression of a synthetic FRB-Draper receptor in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

FRB-Draper INT domain mCherry

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT Shark-mCherry

Plasmid

#126668

Purpose

Inducible expression of Shark-mCherry in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Shark mCherry

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBacHT (His10-4xFRB-SNAP-Lck G2A)

Plasmid

#177887

Purpose

Baculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2A

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Lck (LCK Human)

Use

Tags

10xHis-TEV-4xFRB-SNAP tag

Expression

Insect

Mutation

G2A

Promoter

Polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac(His10-Lckg2a,Y394F)

Plasmid

#177893

Purpose

Baculo expression of His10 tag fused to human LckG2A

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Lck (LCK Human)

Use

Tags

10xHis-TEV

Expression

Insect

Mutation

G2A,Y394F

Promoter

Polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac(His10-Lckg2a,Y394F,Y505F)

Plasmid

#177894

Purpose

Baculo expression of His10 tag fused to human LckG2A

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Lck (LCK Human)

Use

Tags

10xHis-TEV

Expression

Insect

Mutation

G2A,Y394F,Y505F

Promoter

Polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac (GST-SNAP-Shp2)

Plasmid

#177897

Purpose

Baculo expression of GST tag fused to SNAP tag and human Shp2

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

Shp2 (PTPN11 Human)

Use

Tags

TEV-GST-PreScission cut site-SNAP tag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP1

Plasmid

#176690

Purpose

Expression of Chlamydomonas reinhardtii RSP1 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP1

Use

Tags

Expression

Insect

Mutation

None

Promoter

polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP2

Plasmid

#176691

Purpose

Expression of Chlamydomonas reinhardtii RSP2 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP2

Use

Tags

Expression

Insect

Mutation

None

Promoter

polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-GST-TEV-RSP3(160-516)

Plasmid

#176692

Purpose

Expression of Chlamydomonas reinhardtii RSP3 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP3

Use

Tags

GST

Expression

Insect

Mutation

None

Promoter

polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-HISX6-TEV-RSP4

Plasmid

#176693

Purpose

Expression of Chlamydomonas reinhardtii RSP4 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP4

Use

Tags

HISX6

Expression

Insect

Mutation

None

Promoter

polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP5

Plasmid

#176694

Purpose

Expression of Chlamydomonas reinhardtii RSP5 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP5 (RSP5 Chlamydomonas reinhardtii)

Use

Tags

Expression

Insect

Mutation

None

Promoter

polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP6

Plasmid

#176695

Purpose

Expression of Chlamydomonas reinhardtii RSP6 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP6

Use

Tags

Expression

Insect

Mutation

None

Promoter

polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP7

Plasmid

#176696

Purpose

Expression of Chlamydomonas reinhardtii RSP7 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP7

Use

Tags

Expression

Insect

Mutation

None

Promoter

polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP9

Plasmid

#176697

Purpose

Expression of Chlamydomonas reinhardtii RSP9 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP9

Use

Tags

Expression

Insect

Mutation

None

Promoter

polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge