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pDEST17-UbE2E1

Plasmid

#15787

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2E1 (UBE2E1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pTARA

Plasmid

#31491

Depositor

Kathleen Matthews

Article

Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

Insert

RNA polymerase

Use

Tags

Expression

Bacterial

Mutation

N823D

Promoter

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

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mPlum-pBAD

Plasmid

#54564

Purpose

Localization: Protein Expression Vector , Excitation: 590, Emission: 649

Depositor

Michael Davidson, Roger Tsien

Article

Wang et al Proc Natl Acad Sci U S A. 2004 Nov 30;10

Type

Empty backbone

Use

Tags

6xHis and mPlum

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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lacI-PT7-gusA-pBAV1k

Plasmid

#30504

Depositor

Ichiro Matsumura

Article

Murin et al Appl Environ Microbiol. 2011 Oct 21.

Insert

beta-glucoronidase (gusA Escherichia coli)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pET28a-SnoopCatcher

Plasmid

#72322

Purpose

Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a-SnoopCatcher

Use

Tags

His6 tag

Expression

Bacterial

Mutation

changed G842T and D848G of S. pneumoniae adhesin …

Promoter

T7

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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[pSK24] pACYCDuet His-c7orf59|HBXIP

Plasmid

#136143

Purpose

bacterial expression of c7orf59 and HBXIP

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

c7orf59 and HBXIP (LAMTOR4, LAMTOR5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDDGFP-A

Plasmid

#40286

Depositor

Robert Campbell

Article

Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

Insert

ddGFP-A

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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CMV-FlicR1

Plasmid

#74142

Purpose

Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

Depositor

Robert Campbell

Article

Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

Insert

FlicR1

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc

Plasmid

#117728

Purpose

Integration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

NanoLuc

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1 Aquamarine

Plasmid

#106285

Purpose

Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Aquamarine

Use

Tags

6xHis and TEV cleavage (N terminal on insert)

Expression

Bacterial

Mutation

T65S and H148G

Promoter

T7

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pBEL1203

Plasmid

#196024

Purpose

Expression/purification of 6xHis-TEV-MlaC

Depositor

Damian Ekiert, Gira Bhabha

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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R701-X22-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D

Plasmid

#159571

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D (KRAS Human)

Use

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G13D

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pETDuet-1∆HisTag_hNMT_Nef

Plasmid

#66078

Purpose

Single vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli system

Depositor

Dieter Willbold

Article

Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 1

Inserts

NMT (NMT1 Human)
Nef

Use

Tags

Expression

Bacterial

Mutation

Deletion of first 80 amino acids

Promoter

T7-lacO

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBbA2c-RFP

Plasmid

#35326

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pAT28-proD/SaEGFP

Plasmid

#120321

Purpose

Bacterial eGFP labeling

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

eGFP with Staphylococcus aureus codon optimization

Use

Tags

Expression

Bacterial

Mutation

Promoter

proD

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFPV25.1_rpsM_mCerulean3

Plasmid

#124904

Purpose

rpsM promoter (S. typhimurium) driving mCerulean3 (codon-optimized for S. typhimurium)

Depositor

Markus Covert

Article

Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

Insert

rpsM promoter and mCerulean3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-OSK

Plasmid

#136552

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-P2A-Sox2-T2A-Klf4 (Klf4, Sox2, Pou5f1 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pSucCD2

Plasmid

#83324

Purpose

expresses E. coli succinyl-CoA synthetase

Depositor

Robert Hausinger

Insert

sucCD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pKO403_lacZ_Sp

Plasmid

#174726

Purpose

Bifidobacterium-E.coli shuttle vector for seamless gene cloning

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2022 Jan 20;11

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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HSA1_His

Plasmid

#202763

Purpose

human serum albumin design HSA1, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

HSA1

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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