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Plasmid#187620PurposeExpresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertTadA-8e-T7RNAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-2
Plasmid#72677PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterpL promoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-scFv-T
Plasmid#67843PurposepET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.DepositorInsertantibody 1D8 light kappa chain
UseTagsHis-tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pZ8-Ptac
Plasmid#74064PurposeIPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanRDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T7-ARG2
Plasmid#106474PurposeExpresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coliDepositorInsertARG2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGM22082_sfGFP1-10
Plasmid#153515PurposeE.coli expression plasmid for the production of GFP1-10DepositorInsertsfGFP1-10
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE 80L Kan T7WT
Plasmid#102790PurposeExpresses T7 RNA polymeraseDepositorInsertT7 RNA polymerase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 3, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET50_WT MDA5
Plasmid#216802PurposeExpresses wild type (wt) melanoma differentiation-associated protein 5DepositorInsertmelanoma differentiation-associated protein 5 (IFIH1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pProEx-HTa-SOD1 WT
Plasmid#191849Purposeexpress wild-type SOD1 in bacterial cellsDepositorInsertSOD1 (SOD1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDae069 (eMutaT7[PmCDA1] with optimized RBS)
Plasmid#187621PurposeExpresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertPmCDA1-T7RNAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-MKK6-EE
Plasmid#82718PurposeExpresses constitutively active human MKK6. Multiple cloning site 2 is empty.DepositorInsertMKK6 (MAP2K6 Human)
UseTagsExpressionBacterialMutationSer207 to Glu, Thr211 to GluPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIOX
Plasmid#51300PurposeE.coli expression vector for His8-SUMO tagged recombinant proteinDepositorTypeEmpty backboneUseTagsHis8 + SUMOExpressionBacterialMutationPromoterT7Available sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMVV207
Plasmid#215343PurposegRNA expression for S. stutzeri nifL deletion on an RSF1010 backboneDepositorInsertS. stutzeri nifL gRNA
UseCRISPR and Synthetic BiologyTagsNoneExpressionBacterialMutationPromoterJ23119Available sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV205
Plasmid#215342PurposeRecombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ on a BBR1 backboneDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23105Available sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-ELP[V-60]
Plasmid#67013PurposeExpresses malE-ELP[V-60] in E. coliDepositorInsertmalE-ELP[V-60]
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pdCas9
Plasmid#46569PurposeExpresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.DepositorInsertstracrRNA
dcas9
CRISPR array
UseCRISPRTagsExpressionBacterialMutationD10A, H840APromoterAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-X0
Plasmid#115565PurposeControl vector expressing GFP alone without any consensus repeatsDepositorInsertGreen Fluorescent Protein
UseTagsExpressionBacterial and YeastMutationPromoterADH1Available sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
PET-HiFi SpCas9-NLS-6xHis
Plasmid#207376PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertHiFi SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR691APromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only