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Plasmid#116937PurposeBacterial expression vector for 10 sumoDepositorInsert10 SUMO
UseTagsHISExpressionBacterialMutationPromoterT7Available sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
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RBM4_2xRRM-ZNF_pGEX
Plasmid#135154PurposeRecombinant expression of RBM4 with dual-affinity tagDepositorInsertRBM4 (RBM4 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-Int
Plasmid#50000PurposeExpresses the integrase on a suicide plasmidDepositorInsertIntegrase from pMV361
UseTagsExpressionBacterialMutationPromoterownAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPaGE Pyl TAG FliC T248TAG
Plasmid#160089PurposeEncodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.DepositorInsertsPyrrolysyl tRNA(cua) (Methanosarcina mazei)
Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
Flagellin
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Threonine 248 to TAG stop-codon.PromoterPseudomonas aeruginosa Leu-tRNA native promoter a…Available sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
BBa_J72214-BBa_J72090
Plasmid#40782DepositorInsertVioABCDE
UseSynthetic Biology; PhagemidTagsExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
GEF-H1 FLARE212.pa
Plasmid#134930PurposeGEF-H1 biosensor that does not have GEF activity.DepositorInsertGEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
UseTagsNone.ExpressionBacterial and MammalianMutationPromoterCMV and T7Available sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
p80-Katanin-mNeonGreen
Plasmid#191762PurposeExpression of mNeonGreen fused to p80-KataninDepositorInsertp80-Katanin-mNeonGreen (KATNB1 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pProEx RhoA(G14C) CL
Plasmid#224271PurposeProtein purification of RhoA (G14C)DepositorInsertRhoA (RHOA Human)
UseTagsExpressionBacterialMutationG14CPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pProEx Rheb(R15C) CL
Plasmid#224266PurposeProtein purification of Rheb (R15C)DepositorInsertRheb (RHEB Human)
UseTagsExpressionBacterialMutationR15CPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET11d-RAN
Plasmid#108919PurposeExpresses human RANDepositorInsertRAN (RAN Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a-SUMO1
Plasmid#53138PurposeE.coli expression of untagged mature human SUMO1 (amino acids 1-97)DepositorInsertSUMO1 (SUMO1 Human)
UseTagsExpressionBacterialMutationmature form (amino acids 1-97)PromoterT7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a(ICOS)
Plasmid#177903PurposeBacterial expression of his10-tagged human ICOS Intracellular DomainDepositorInsertICOS (aa 162-199 only) (ICOS Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLIC-EcTOP
Plasmid#224463PurposeExpression of recombinant Escherichia coli topoisomerase IDepositorInsertEscherichia coli TopA
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
proinsulin-msGFP2 (C48S C70S)
Plasmid#218956PurposeExpresses proinsulin fused to msGFP2 (C48S C70S) in bacteriaDepositorInsertproinsulin-msGFP2 C48S C70S
UseTagsproinsulinExpressionBacterialMutationPromoterT5Available sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSNRW-Z5-Nluc
Plasmid#174487PurposeRecombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.DepositorInsertZ5-Nluc
UseTags6x Histidine tag (N terminal on insert)ExpressionBacterialMutationPromoterT7lac promoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Human_H2B.1
Plasmid#42630DepositorInsertHuman H2B.1 (H2BC8 Human)
UseTagsUntaggedExpressionBacterialMutationPromoterT7Available sinceFeb. 22, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDest-565
Plasmid#11520PurposeGateway destination vector for bacterial expression; His,GST tagDepositorTypeEmpty backboneUseGateway destination vectorTagsHis6-GSTExpressionBacterialMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-puro-kozak-scFv-GCN4-HAtag-msfGFP(V206K)-GBI
Plasmid#205162Purposeexpress scFv-GCN4-HAtag-msfGFP(V206K)-GBIDepositorInsertscFv-GCN4-HAtag-msfGFP(V206K)-GBI
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p1-GST-ArcFL
Plasmid#119877PurposeBacterial Expression construct for protein production and purificationDepositorInsertActivity Regulated Cytoskeletal Protein (Arc Rat)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only