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Addgene

BBa_J72214-BBa_J72090
(Plasmid #40782)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40782 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    p15a, CamR, phagemid
  • Vector type
    Bacterial Expression, Synthetic Biology ; phagemid

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Growth instructions
    This plasmid will turn the strain carrying it a deep purple (more purple the older the colonies are).
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VioABCDE
  • Insert Size (bp)
    7345

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For detailed composition information, look up the backbone (BBa_J72114) and insert (BBa_J72090) on the Registry of Standard Biological Parts (partsregistry.org)

Please note that Addgene's sequencing results indicate the VioD gene is absent from this construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BBa_J72214-BBa_J72090 was a gift from Christopher Anderson (Addgene plasmid # 40782 ; http://n2t.net/addgene:40782 ; RRID:Addgene_40782)
  • For your References section:

    Scalable plasmid transfer using engineered P1-based phagemids. Kittleson JT, DeLoache W, Cheng HY, Anderson JC. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30. 10.1021/sb300054p PubMed 23656280