-
Plasmid#50448PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylaseDepositorInsertPPCS PPCDC
UseTags6XHIS tagExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pGEX4T-1 Srap1
Plasmid#89691PurposeExpresses murine Srap1 in bacteriaDepositorInsertMurine SRAP domain ortholog Srap1 (Hmces Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
MK13_HUMAN_D0
Plasmid#79693PurposeThis plasmid encodes the kinase domain of MK13. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMK13
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_R43W_E93G
Plasmid#109406PurposeRepA MutantDepositorInsertrepA
UseTagsRFPExpressionBacterialMutationR43W and E93G repA mutations to increase copy num…PromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMP1290
Plasmid#119267Purposeconstruction intermediateDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_ygiF-CHAD
Plasmid#127793PurposeExpresses the CHAD domain of ygiF in E. coli cellsDepositorInsertygiF-CHAD (ygiF Escherichia coli)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationN-terminal TTM domain of ygiF (residues 201-433)PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pGEX4T1-NDFIP2
Plasmid#179330PurposeE. coli optimized expression of GST-fusion of human NDFIP2DepositorInsertNDFIP2 (NDFIP2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHL 636
Plasmid#53015PurposeAmpR + PLtetO-1::RBS (st7) fimE::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1DepositorInsertsfimE
fimB
UseSynthetic BiologyTagsAsp terminator, RBS(st7), and T1 terminatorExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGMCZq17-OXOX
Plasmid#31289DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pXL-BACII-attPGAL4LWL
Plasmid#26260DepositorInsertGAL4 (GAL4 Budding Yeast)
UseDrosophila transgenesisTagsExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFLIPmal-40uDelta1YFP
Plasmid#18853DepositorInsertoptimized maltose FRET sensor
UseTagsCFP and YFPExpressionBacterialMutationN227A;PromoterAvailable sinceOct. 23, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJL1-EPA-DNNNS-DQNRT
Plasmid#128397PurposeIn vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sitesDepositorInsertExotoxin A
UseTags6xHis tagExpressionBacterialMutation242DNNNS246 and 384DQNRT388 engineered glycosylat…PromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO BMI
Plasmid#69796PurposeGateway entry vector for BMIDepositorInsertBMI (BMI1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
pDL14
Plasmid#106901PurposepDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent mannerDepositorInsertsgam
bet
mutS
lacI
rhaR
rhaS
UseTagsExpressionBacterialMutationLysine 622 was replaced by AlaninePromoterAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_XRCC4_XRCC4
Plasmid#110047PurposeProtein expression and purification of XRCC4_XRCC4DepositorInsertXRCC4_XRCC4 (XRCC4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
pRAV12
Plasmid#67713PurposeParental plasmid for transcription of RNAs with a HdV/M-domain 3'-tag for native purificationDepositorInsertTranscription cassette for 3'-tagged RNAs
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7 RNA polymeraseAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only