pFLIPmal-40uDelta1YFP
(Plasmid
#18853)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 18853 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSET
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameoptimized maltose FRET sensor
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SpeciesE. coli
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MutationN227A;
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Tags
/ Fusion Proteins
- CFP (N terminal on insert)
- YFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
W230A mutation, which we introduced to change the affinity.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFLIPmal-40uDelta1YFP was a gift from Wolf Frommer (Addgene plasmid # 18853 ; http://n2t.net/addgene:18853 ; RRID:Addgene_18853) -
For your References section:
Fluorescence Resonance Energy Transfer sensors for quantitative monitoring of pentose and disaccharide accumulation in bacteria. Kaper T, Lager I, Looger LL, Chermak D, Frommer WB. Biotechnol Biofuels. 2008 Jun 3. 1(1):11. 10.1186/1754-6834-1-11 PubMed 18522753