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Addgene

pRAV12
(Plasmid #67713)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67713 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 2800
  • Modifications to backbone
    insertion between EcoRI and HindIII sites of the MCS
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Transcription cassette for 3'-tagged RNAs
  • Species
    Synthetic
  • Insert Size (bp)
    500
  • Promoter T7 RNA polymerase

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HinDIII (not destroyed)
  • 5′ sequencing primer M13-F20
  • 3′ sequencing primer M13-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRAV12 was a gift from Robert Batey & Jeffrey Kieft (Addgene plasmid # 67713 ; http://n2t.net/addgene:67713 ; RRID:Addgene_67713)
  • For your References section:

    A general method for rapid and nondenaturing purification of RNAs. Kieft JS, Batey RT. RNA. 2004 Jun;10(6):988-95. PubMed 15146082