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pSFBAD09

Plasmid

#11963

Depositor

Hideo Iwai

Article

Zuger et al Nat Biotechnol. 2005 Jun . 23(6):736-40.

Insert

SspDnaEC

Use

Tags

CBD

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 21, 2007

Availability

Academic Institutions and Nonprofits only

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18 pCOLI_A_Trx

Plasmid

#160255

Purpose

N-term His-Thioredoxin 3C cleavable Amp resistant bacterial expression vector

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Use

Tags

6xHis and Thioredoxin

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pTHS

Plasmid

#166938

Purpose

Toehold switch multi-level gene expression controller (MLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Genetic circuit expressing GFP- Toehold switch

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PTAC

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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3CEG

Plasmid

#60107

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

BIRC6:SDC136-F10:C41228 (BIRC6 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pOSV812

Plasmid

#126605

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Article

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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NEDD4

Plasmid

#25141

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

nedd4.0001.0152 (NEDD4 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pET28a-TmCA-P(11)4

Plasmid

#185783

Purpose

Encodes thermostable carbonic anhydrase enzyme linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

TmCA-P(11)4

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-BCA-Q11

Plasmid

#185781

Purpose

Encodes enzyme bovine carbonic anhydrase linked to Q11 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

BCA-Q11

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046879

Plasmid

#149238

Purpose

Bacterial expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

May 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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T7-PseB-His6

Plasmid

#89723

Purpose

Expresses PseB from C. jejuni with an N-terminal T7 tag and a C-terminal His6 tag

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

PseB

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBA1018

Plasmid

#189587

Purpose

T4wtGT7 deletion HR vector 250bp HR Arms (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

T4wtGT7 Deletion Locus 250bp Homology Arms

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Encodes for a full deletion of T4 from 52.1 (part…

Promoter

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBXNH3CA_MBP

Plasmid

#132700

Purpose

expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Maltose binding Protein

Use

Tags

Avi and His

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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p3034 GST-HPV-11 E6

Plasmid

#10848

Depositor

Peter Howley

Article

Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

Insert

HPV11 E6

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
pMBP-HIS2-Vps20_deltaC

Plasmid

#21491

Depositor

James Hurley

Article

Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

Insert

Vps20 Delta_C (VPS20 Budding Yeast)

Use

Tags

6x His and MBP

Expression

Bacterial

Mutation

bp 511-663 deleted

Promoter

Available since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pRset-ARSeNL

Plasmid

#207872

Purpose

Bacterial expression of the BRET ATP sensor

Depositor

Mathew Tantama

Article

Min et al Sensors (Basel). 2019 Aug 10;19(16). pii

Insert

ARSeNL

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBLlSh2

Plasmid

#170981

Purpose

Expression of ShCAST under control of lacI Plac promoter/repressor pair.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Inserts

lacI
ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlac01

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-27

Plasmid

#172752

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-27

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP1-C

Plasmid

#128311

Purpose

Express hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pFAB3893

Plasmid

#47844

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 13 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 13 and BCD1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

see insert

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pBAT4

Plasmid

#112580

Purpose

Used for expression of protein in E. coli as non-fusions

Depositor

Marko Hyvönen, Johan Peränen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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