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Plasmid#21505DepositorInsertTsg101 (TSG101 Human)
UseTagsCherry*ExpressionBacterialMutationnonePromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pet28a-His6-Keap1
Plasmid#62454PurposeHis6 tagged Keap1 for E.coli expressionDepositorInsertKeap1 (KEAP1 Human)
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSR
Plasmid#161983PurposeAllele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile 630. Uses inducible expression of CD2517.1 toxin as a counter-selection markerDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTP1174
Plasmid#104162PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0-6H-TEV-KRAS G12D
Plasmid#159439PurposeExpresses KRAS G12D in E.coliDepositorInsertKRAS G12D (KRAS Human)
UseTags6xHISExpressionBacterialMutationPromoterT5Available sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBIS
Plasmid#17817DepositorInsertmevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
AviTag-SpyCatcher
Plasmid#72326PurposeBacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.DepositorInsertAviTag-SpyCatcher
UseTagsN-term His6 tag and TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGR
Plasmid#46002PurposeReporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinoseDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PFO
Plasmid#92109PurposeRecombinant expression in E.ColiDepositorInsertPFO
UseTags6xHisExpressionBacterialMutationDelta27aaPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)
Plasmid#199605PurposeExpression of cell permeable opAsCas12a in E. Coli.DepositorInsertopCas12a-T8N
UseCRISPRTags6xHis, Twin-strep, SUMOExpressionBacterialMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-AsnRS
Plasmid#124105PurposeBacterial expression plasmid for PURE system componentsDepositorInsertAsparaginyl-tRNA ligase (asnS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET16b-His-ArgRS
Plasmid#124104PurposeBacterial expression plasmid for PURE system componentsDepositorInsertArgin+I4 ligase (argS Escherichia coli)
UseTags10x-HisExpressionBacterialMutationPromoterAvailable sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBJ2
Plasmid#167131PurposeA gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoterDepositorInsertluxCDABE
UseLuciferaseTagsExpressionBacterialMutationPromoterKanamycin promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GlnRS-His
Plasmid#124108PurposeBacterial expression plasmid for PURE system componentsDepositorInsertGlutaminyl-tRNA ligase (glnS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2
Plasmid#205160Purposeexpress β-ACTIN-3′UTR-8xMS2DepositorInsertβ-ACTIN-3′UTR-24xMS2
UseTagstagBFPExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐hsRNASEH2BCA
Plasmid#108692PurposeFor expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzymeDepositorUseTagsGSTExpressionBacterialMutationShine Dalgarno sequence upstream of start codon a…PromotertacAvailable sinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMo168
Plasmid#27389DepositorInsertpMo168
UseTagsExpressionBacterialMutationA replicative vector for gene expression in Burkh…PromoterAvailable sinceMarch 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMEG_YLCas9_empty
Plasmid#117826PurposeCRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolyticaDepositorInsertYlpTEF_hCas9_YlCyc1TT
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBJ1
Plasmid#167130PurposeA gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoterDepositorInsertluxCDABE
UseLuciferaseTagsExpressionBacterialMutationPromoterFrr promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBJ4
Plasmid#167133PurposeA kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoterDepositorInsertluxCDABE
UseLuciferaseTagsExpressionBacterialMutationPromoterKanamycin promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only