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Addgene

pGR
(Plasmid #46002)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46002 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSB1A10
  • Backbone manufacturer
    http://partsregistry.org/partsdb/get_part.cgi?part=pSB1A10
  • Backbone size (bp) 5191
  • Modifications to backbone
    Removed RNase E sites and the hairpin in front of mRFP1
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Promoter PBAD

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Registry of Standard Biological Parts: http://partsregistry.org/partsdb/get_part.cgi?part=pSB1A10
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a reporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinose.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGR was a gift from Christopher Voigt (Addgene plasmid # 46002 ; http://n2t.net/addgene:46002 ; RRID:Addgene_46002)
  • For your References section:

    Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Chen YJ, Liu P, Nielsen AA, Brophy JA, Clancy K, Peterson T, Voigt CA. Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515. 10.1038/nmeth.2515 PubMed 23727987