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pmCellFree_KA2_ZIKV PrM
Plasmid#145360PurposeCell-free/mammalian expression vector for Zika virus PrM protein with N-term mCherryDepositorInsertZIKV PrM
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceMarch 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEM7070
Plasmid#214008PurposePrpsM driven expression of ssrA-tagged mNeonGreen (ASV)DepositorInsertmNeonGreen-ASV
UseTagsExpressionBacterialMutationPromoterPrpsMAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEM7074
Plasmid#214012PurposePrpsM driven expression of ssrA-tagged mCerulean (ASV)DepositorInsertmCerulean-ASV
UseTagsExpressionBacterialMutationPromoterPrpsMAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEM7068
Plasmid#214006PurposePrpsM driven expression of ssrA-tagged eGFP (ASV)DepositorInserteGFP-ASV
UseTagsExpressionBacterialMutationPromoterPrpsMAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136464
Plasmid#149534PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEVExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Twitch-2C pcDNA3
Plasmid#104501Purposefluorescent reporter for calcium signalingDepositorInsertTwitch-2C
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
KM-CashGem-Xyl2-URA3-LINEAR
Plasmid#174838PurposepRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianusDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastMutationPromoterTEF1pAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcrRNA.con
Plasmid#61285PurposeConstitutive repeat-only E. coli CRISPR arrayDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
4P7A
Plasmid#61954PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMLH1:JMC01L-F09:C205690 (MLH1 Human)
UseTags6xHis and MHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available sinceMarch 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
p15A-OptoCre-cat-T172A-P-R
Plasmid#189854PurposeCre-inducible activation of cat-T172A for chloramphenicol resistance.DepositorInsertP-loxP-TT-loxP-R-catT172A
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP (T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15TV-L-hlPYK WT
Plasmid#187975PurposeExpresses the human liver pyruvate kinaseDepositorInserthlPYK WT
UseTagsHexahistagExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti
Plasmid#115795PurposeExpresses AcrIIA4 in human cells, contained in a lentiviral vector.DepositorInsertAcrIIA4
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterEF-1a promoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET empty polycistronic destination vector (2Z)
Plasmid#29776DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEB2-TurboRFP
Plasmid#103996PurposePlasmid encoding TurboRFPDepositorInsertTurboRFP
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP578 (R32G, T68A, L79F, …PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACYC.GFP1Amb
Plasmid#102792PurposeExpresses GFP with a premature stop codonDepositorInsertGreen Fluorescent Protein
UseTagsExpressionBacterialMutationY39StopPromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCURE
Plasmid#23013DepositorInsertI-CreI homing endonuclease
UseTagsExpressionBacterialMutationI-CreI expression is controlled by the arabinose …PromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGM202T7
Plasmid#69993PurposeE. coli/Streptomyces expression vector containing the T7-promoterDepositorInsertsaphII
tsr
rep
sso
T7 promoter
dso
His-Orf
UseTagsN-terminal and C-terminal His tag encoding sequen…ExpressionBacterialMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-FGF21
Plasmid#182407PurposeBacterial expression of Vesicle Nucleating peptide-fgf21 fusionDepositorInsertVNp-fgf21 (FGF21 Human)
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only