Showing: 8501 - 8520 of 27133 results
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Plasmid#101718PurposeK10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertddFLN4 fingerprint, K10 peptide (KRT10 Dictyostelium discoideum, Human)
UseTagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-HIS-ddFLN4-Fgß
Plasmid#101719Purposewildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forcesDepositorInsertddFLN4 fingerprint, Fgß peptide (FGB Dictyostelium discoideum, Human)
UseTagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pWUR790
Plasmid#101723PurposeSynthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgoDepositorInsertPfAgo
UseTagsStrep(II)-tagExpressionBacterialMutationPromoterT7 PromotorAvailabilityAcademic Institutions and Nonprofits only -
pSCB-YFP
Plasmid#101737PurposeThe plasmid can be either plasmid propogation backbone or expression vector in specific strainDepositorTypeEmpty backboneUseSynthetic BiologyTagseYFPExpressionBacterialMutationPromoterTrc1OAvailabilityAcademic Institutions and Nonprofits only -
pAK22
Plasmid#101741Purposeencoding two copies of T. thermophilus SecG for expression of SecYEG complexDepositorInsertThermus thermophilus HB8 SecG x 2
UseTagsNONEExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pTT610
Plasmid#101742Purposeencoding SecY(L2V, R252G)–(GGSG)4–SecA1-939–His10 fusion protein and SecE and SecG from Thermus thermophilus HB8DepositorUseTagsHis10ExpressionBacterialMutationSecY(L2V, R252G)–(GGSG)4–SecA1-939–His10 fusion p…PromoterLacAvailabilityAcademic Institutions and Nonprofits only -
pET28a-FgßF3-ddFLN4-HIS-ybbr
Plasmid#101743Purposemutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertFgßF3 peptide, ddFLN4 fingerprint (FGB Dictyostelium discoideum, Human)
UseTagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4, added 9F in FgßF3PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pMH-MBP
Plasmid#101768PurposeGateway-compatible destination vector for bacterial expression of N-terminal MBP-tag fusion proteinDepositorTypeEmpty backboneUseTagsMBPExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
H14_MBP_SUMO-Cys-linker-BAF
Plasmid#101777PurposeExpresses His-MBP-tagged BAF in E.coli (SUMO-cleavable)DepositorInsertBAF (BANF1) (BANF1 Human)
UseTagsHis14-MBP-SUMOExpressionBacterialMutationPromoterT5AvailabilityAcademic Institutions and Nonprofits only -
H14-MBP-SUMO-FLAG-LEM
Plasmid#101778PurposeExpresses His-MBP-tagged mutant LEM-domain (FLAG-tagged) in E.coli (SUMO-cleavable)DepositorInsertMAN1 (LEMD3 Human)
UseTagsHis14-MBP-SUMO-FLAGExpressionBacterialMutationamino acids 6-51 of human MAN1 (LEM domain)PromoterT5AvailabilityAcademic Institutions and Nonprofits only -
H14-MBP-SUMO-Cys-Linker-BAF_G47E
Plasmid#101779PurposeExpresses His-MBP-tagged mutant BAF (G47E) in E.coli (SUMO-cleavable)DepositorInsertBAF (BANF1) (BANF1 Human)
UseTagsHis14-MBP-SUMOExpressionBacterialMutationG47E mutationPromoterT5AvailabilityAcademic Institutions and Nonprofits only -
H14-MBP-SUMO-Cys-Linker-BAF_L58R
Plasmid#101780PurposeExpresses His-MBP-tagged mutant BAF (L58R) in E.coli (SUMO-cleavable)DepositorInsertBAF (BANF1) (BANF1 Human)
UseTagsHis14-MBP-SUMOExpressionBacterialMutationL58R mutationPromoterT5AvailabilityAcademic Institutions and Nonprofits only -
pET28a-R4-uAb
Plasmid#101801PurposeBacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAbDepositorInsertR4-uAb
UseTags6xHis and FlagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET21 gamma-tubulin
Plasmid#101825Purposeproduction of gamma-tubulin in bacteriaDepositorInsertgamma-tubulin 1 (TUBG1 Human)
UseTagsHisExpressionBacterialMutationWhole proteinPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pTO_CHMP2B_LAP
Plasmid#101848PurposeTet-inducible expression of human CHMP2B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integrationDepositorInsertCHMP2B (CHMP2B Human)
UseFlp-frt recombination, tet-inducible expressionTagsLocalization and Affinity Purification (LAP) tagExpressionBacterial and MammalianMutationsilent mutation in Thr 104; A to TPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pTO_CHMP3_LAP
Plasmid#101849PurposeTet-inducible expression of human CHMP3 with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integrationDepositorInsertCharged Multivesicular Body Protein 3 (CHMP3 Human)
UseGateway cloning, flp-frt recombination, tet-induc…TagsLocalization and Affinity Purification (LAP) tagExpressionBacterial and MammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pTO_CHMP4B_LAP
Plasmid#101850PurposeTet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integrationDepositorInsertCharged Multivesicular Body Protein 4B (CHMP4B Human)
UseGateway cloning, flp-frt recombination, tet-induc…TagsLocalization and Affinity Purification (LAP) tagExpressionBacterial and MammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pTO_Snf7_LAP
Plasmid#101851PurposeTet-inducible expression of Snf7 from S. cerevisiae with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integrationDepositorInsertSNF7 (SNF7 Budding Yeast)
UseGateway cloning, flp-frt recombination, tet-induc…TagsLocalization and Affinity Purification (LAP) tagExpressionBacterial and MammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
5JJZ (CDYL2)
Plasmid#101876PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCDYL2 (CDYL2 Human)
UseTags6x His and AviTagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
5KO9 (HMCES)
Plasmid#101877PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHMCES (HMCES Human)
UseTags6x His (modified C-term tag is AENLYFQSHHHHHH)ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only
Showing: 8501 - 8520 of 27133 results