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pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti
(Plasmid #115795)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115795 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCF525 (SIN-LTR lenti)
  • Backbone manufacturer
    N/A
  • Backbone size w/o insert (bp) 8100
  • Total vector size (bp) 8376
  • Vector type
    Mammalian Expression, Bacterial Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AcrIIA4
  • Species
    Listeria monocytogenes
  • Insert Size (bp)
    264
  • GenBank ID
    WP_003723290.1
  • Promoter EF-1a promoter

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti was a gift from Jennifer Doudna (Addgene plasmid # 115795 ; http://n2t.net/addgene:115795 ; RRID:Addgene_115795)

  • For your References section:

    Systematic discovery of natural CRISPR-Cas12a inhibitors. Watters KE, Fellmann C, Bai HB, Ren SM, Doudna JA. Science. 2018 Oct 12;362(6411):236-239. doi: 10.1126/science.aau5138. Epub 2018 Sep 6. 10.1126/science.aau5138 PubMed 30190307
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