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Plasmid#113318PurposeAraC-pBAD-dCas9, MCP-SoxS_R93ADepositorInsertdCas9 and MCP-SoxS_R93A
UseCRISPRTagsExpressionBacterialMutationR93APromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
BBa_J72114-BBa_J72100
Plasmid#40781DepositorInsertlacZ
UseSynthetic Biology; PhagemidTagsExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-3xMyc
Plasmid#129569PurposeEmpty backbone for Myc TagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSrpR-S2
Plasmid#74692PurposeSrpR-S2 gateDepositorInsertPTac-RBSS2-SrpR-PSrpR-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTn7xKS-sfGFP
Plasmid#117394PurposeTn7 tagging vector pTn7xKS with sfGFP expression scaffoldDepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtacAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-hsGFP
Plasmid#64911PurposeEncodes a Fluorescent Reporter for Hydrogen Sulfide in E. ColiDepositorInserthsGFP
UseTagsHis TagExpressionBacterialMutationChromophore tyrosine of the protein mutated to TA…PromoteraraBADAvailable sinceMay 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-EsaI
Plasmid#47660PurposeesaI gene downstream of esaR in pAC-EsaR (CmR)DepositorInsertesaI-term
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+eGFP-bromo5
Plasmid#157804PurposeExpression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1DepositorInserteGFP-bromo5
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-51
Plasmid#79966PurposeLate1-O3-33-myc-PHDepositorInsertEPN-51
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDFNKI-strepII-3C-LIC-strep
Plasmid#108708PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsStrepII-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1-hPXR-LBD
Plasmid#67769PurposeExpression GST-hPXR-LBD in bacteriaDepositorInserthuman pregnane X receptor (PXR) ligand binding domain (NR1I2 Human)
UseTagsGSTExpressionBacterialMutationContains amino acids 107 to 434 of human PXRPromoterTacAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET51b_HCAII
Plasmid#187110PurposeExpression of HCAII in bacteriaDepositorInsertHCAII
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_PEX14_Pex14
Plasmid#178484PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertPEX14_Pex14 (PEX14 Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX Txk-SH2
Plasmid#46526DepositorInsertTxk (Txk Mouse)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 138-258PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX RASGAP(N)-SH2
Plasmid#46432DepositorInsertGap/RASGAP(N) (RASA1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 168-283PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
mOrange in pBSDONR P4r-P2
Plasmid#121529PurposeFor making C-terminal mOrange fusions using multisite Gateway cloningDepositorInsertmOrange
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsNot4-GB1His6_AG
Plasmid#148792PurposeBacterial Expression of HsNot4DepositorInsertHsNot4 (CNOT4 Human)
UseTagsExpressionBacterialMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRD395
Plasmid#163102PurposeExpression of mCherry_H-NSdbd in Escherichia coli (and, potentially, other bacteria)DepositorInsertmCherry_H-NSdbd
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
DARS_pNIC-Bio3
Plasmid#153048PurposeExpression construct for producing Avi tagged Glycyl-tRNA synthetaseDepositorInsertDARS (DARS1 Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVRb03_up1198
Plasmid#49706DepositorInsertsfgfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPecf03_up1198Available sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only