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Addgene

pGEX-6P1-hPXR-LBD
(Plasmid #67769)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67769 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX-6P1
  • Backbone size w/o insert (bp) 4984
  • Total vector size (bp) 5968
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human pregnane X receptor (PXR) ligand binding domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    984
  • Mutation
    Contains amino acids 107 to 434 of human PXR
  • GenBank ID
    NM_003889.3 NM_003889.3
  • Entrez Gene
    NR1I2 (a.k.a. BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, PXR, SAR, SXR)
  • Promoter Tac
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer pGEX Forward Primer
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-6P1-hPXR-LBD was a gift from Sridhar Mani (Addgene plasmid # 67769 ; http://n2t.net/addgene:67769 ; RRID:Addgene_67769)
  • For your References section:

    Novel yeast-based strategy unveils antagonist binding regions on the nuclear xenobiotic receptor PXR. Li H, Redinbo MR, Venkatesh M, Ekins S, Chaudhry A, Bloch N, Negassa A, Mukherjee P, Kalpana G, Mani S. J Biol Chem. 2013 May 10;288(19):13655-68. doi: 10.1074/jbc.M113.455485. Epub 2013 Mar 22. 10.1074/jbc.M113.455485 PubMed 23525103