pGEX-6P1-hPXR-LBD
(Plasmid
#67769)
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PurposeExpression GST-hPXR-LBD in bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 67769 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-6P1
- Backbone size w/o insert (bp) 4984
- Total vector size (bp) 5968
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman pregnane X receptor (PXR) ligand binding domain
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SpeciesH. sapiens (human)
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Insert Size (bp)984
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MutationContains amino acids 107 to 434 of human PXR
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GenBank IDNM_003889.3 NM_003889.3
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Entrez GeneNR1I2 (a.k.a. BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, PXR, SAR, SXR)
- Promoter Tac
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer pGEX Forward Primer (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-6P1-hPXR-LBD was a gift from Sridhar Mani (Addgene plasmid # 67769 ; http://n2t.net/addgene:67769 ; RRID:Addgene_67769) -
For your References section:
Novel yeast-based strategy unveils antagonist binding regions on the nuclear xenobiotic receptor PXR. Li H, Redinbo MR, Venkatesh M, Ekins S, Chaudhry A, Bloch N, Negassa A, Mukherjee P, Kalpana G, Mani S. J Biol Chem. 2013 May 10;288(19):13655-68. doi: 10.1074/jbc.M113.455485. Epub 2013 Mar 22. 10.1074/jbc.M113.455485 PubMed 23525103