Skip to main content
Addgene
Showing: 61 - 80 of 28286 results

  4. pYDE009

    Plasmid
    #194151
    Purpose
    Delivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.
    Depositor
    Edward Geisinger
    Article
    Bai et al J Bacteriol. 2021 May 20;203(12):e005652
    Insert
    tetR-tetP-dcas9-rrnB T1-T7Te
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tetp
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    194151-plasmid-map-sequence-id-382447

    Enlarge

  5. pJMP3664

    Plasmid
    #222350
    Purpose
    Expression vector with PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.
    Depositor
    Jason Peters
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222350-plasmid-map-sequence-id-439070

    Enlarge

  6. pJMP12049

    Plasmid
    #222355
    Purpose
    Tn7 integrative vector with GFP expressed under PabstBR promoter and aprR marker on Tn7 transposon.
    Depositor
    Jason Peters
    Insert
    sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PabstBR
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222355-plasmid-map-sequence-id-439062

    Enlarge

  7. pJE53

    Plasmid
    #194153
    Purpose
    sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.
    Depositor
    Edward Geisinger
    Article
    Bai et al J Bacteriol. 2021 May 20;203(12):e005652
    Insert
    sgRNA with mrfp control guide sequence
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    194153-plasmid-map-sequence-id-382435

    Enlarge

  9. pYDE007

    Plasmid
    #194152
    Purpose
    sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, CarbR. Contains mrfp nontargeting control guide sequence.
    Depositor
    Edward Geisinger
    Article
    Bai et al J Bacteriol. 2021 May 20;203(12):e005652
    Insert
    sgRNA with mrfp control guide sequence
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    194152-plasmid-map-sequence-id-382443

    Enlarge

  11. pCarCBADE_ProB(I69E)A

    Plasmid
    #117756
    Purpose
    ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Article
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLac(UV5)
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    117756-plasmid-map-sequence-id-230175

    Enlarge

  18. T4 Lysozyme S38D L99A M102Q N144D

    Plasmid
    #18477
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    S38D L99A M102Q N144D
    Promoter
    Available since
    Sept. 15, 2008
    Availability
    Academic Institutions and Nonprofits only

  20. mu-IFNg-pCIneo

    Plasmid
    #163517
    Purpose
    Expresses murine IFN gamma in mammalian cells
    Depositor
    Thomas Weber
    Insert
    murine-IFN gamma (Ifng Mouse)
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163517-plasmid-map-sequence-id-321578

    Enlarge

Showing: 61 - 80 of 28286 results