-
Plasmid#40235DepositorInsertyEGFP1
UseTagsHIS3MX6ExpressionBacterialMutationPromoterGAL1Available sinceOct. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)::NL3F10H
Plasmid#141290PurposeNLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC3F10H
UseLuciferaseTagsFlag (x3) and His (x10)ExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM1s3ATScsg-Etag
Plasmid#137946PurposeE. coli Nissle pMUT1-derived plasmid with ampicillin resistance and temperature sensitive curli expressionDepositorInsertsSynthetic curli operon
Synthetic curli operon
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterpTlpA36Available sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbE5a-Opto-Cre-Vvd
Plasmid#134404PurposeLight-inducible Cre recombinase split with Vivid photodimers for bacterial expression.DepositorInsertOpto-Cre-Vvd
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-knt-P-R
Plasmid#189849PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP-loxP-TT-loxP-R-knt
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP (T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_Sensor_27B_LacZ
Plasmid#75006PurposeToehold switch sensor to detect Zika VirusDepositorInsertZIKV_sensor_27B_LacZ
UseTagsLacZExpressionBacterialMutationPromoterT7Available sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1415
Plasmid#84828PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 intronsDepositorInsertC. elegans codon optimized GFP
UseTagsExpressionBacterial and WormMutationPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b-pA-Mnase
Plasmid#124883PurposeTo transform BL21-Gold(DE3) and induced by IPTG for His-proteinA-MNase fusion protein.DepositorInsertprotein A-MNase
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO ALIX Bro1-V (1-702)
Plasmid#80642PurposeResidues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable N-term His-tag; Sundquist Lab Internal ID: WISP10-648DepositorInsertALIX Bro1-V (PDCD6IP Human)
UseTagsHis TagExpressionBacterialMutationResidues 1-702PromoterAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28b-pchG WT
Plasmid#87107Purposeexpresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosaDepositorInsertpchG WT
UseTagshis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - TR
Plasmid#89621Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG inductionDepositorInsertThioredoxin
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-irp3 WT
Plasmid#87106Purposeexpresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocoliticaDepositorInsertirp3 WT
UseTagshis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1320
Plasmid#84829PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 intronsDepositorInsertC. elegans codon optimized GFP
UseTagsExpressionBacterial and WormMutationPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pReporter_12
Plasmid#60572PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_12
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-knt-P**-R
Plasmid#189851PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP**-loxP-TT-loxP-R-knt
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP** (modified T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-cat-P-R
Plasmid#189853PurposeCre-inducible activation of cat for chloramphenicol resistance.DepositorInsertP-loxP-TT-loxP-R-cat
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP (T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPPC009.106
Plasmid#171142PurposeExpression of J106 scRNA on pBBR1-KmR plasmidDepositorInsertJ106 scRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a - NR
Plasmid#89625PurposeExpresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG inductionDepositorInsertNitrate reductase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
PfICH pET15b
Plasmid#60684PurposeRecombinant protein expression of Isocyanide Hydratase from Pseudomonas fluorescensDepositorInsertinhA
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only